Tracking bla_CTX-M transmission through transposable elements in uropathogenic and commensal E. coli.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-01-29 DOI:10.1080/17460913.2025.2459526

Denyss Guilcazo, Liseth Salinas, Cristina Chavez, Katherine Vasquez, Gabriela I Mendez, Lance B Price, Jay P Graham, Joseph N S Eisenberg, Gabriel Trueba

{"title":"Tracking blaCTX-M transmission through transposable elements in uropathogenic and commensal E. coli.","authors":"Denyss Guilcazo, Liseth Salinas, Cristina Chavez, Katherine Vasquez, Gabriela I Mendez, Lance B Price, Jay P Graham, Joseph N S Eisenberg, Gabriel Trueba","doi":"10.1080/17460913.2025.2459526","DOIUrl":null,"url":null,"abstract":"Aim: To investigate the nucleotide sequences associated with transposable elements carrying blaCTX-M allelic variants as potential markers for the transmission of antimicrobial resistance genes between domestic animals, humans and the environment.Materials & methods: We conducted whole-genome sequencing and analyzed the nucleotide sequences of most abundant blaCTX-M allelic variants (blaCTX-M-27, blaCTX-M-55, and blaCTX-M-65) in commensal Escherichia coli (n = 20) from household members in Quito and uropathogenic E. coli (UPEC) (n = 149) isolated from nine clinics in Quito, Ecuador.Results: The Ecuadorian commensal E. coli and UPEC displayed identical nucleotide sequences surrounding the blaCTX-M gene and the synteny was similar to those found in other parts of the world; however phylogenetic analysis indicated that the genetic environments in Ecuadorian isolates were unique.Conclusion: These findings suggest that the nucleotide sequences flanking the blaCTX-M genes may be useful for resolving ARG transmission pathways, especially inter-regional analyses.","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"1-7"},"PeriodicalIF":2.5000,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/17460913.2025.2459526","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aim: To investigate the nucleotide sequences associated with transposable elements carrying bla_CTX-M allelic variants as potential markers for the transmission of antimicrobial resistance genes between domestic animals, humans and the environment.

Materials & methods: We conducted whole-genome sequencing and analyzed the nucleotide sequences of most abundant bla_CTX-M allelic variants (bla_CTX-M-27, bla_CTX-M-55, and bla_CTX-M-65) in commensal Escherichia coli (n = 20) from household members in Quito and uropathogenic E. coli (UPEC) (n = 149) isolated from nine clinics in Quito, Ecuador.

Results: The Ecuadorian commensal E. coli and UPEC displayed identical nucleotide sequences surrounding the bla_CTX-M gene and the synteny was similar to those found in other parts of the world; however phylogenetic analysis indicated that the genetic environments in Ecuadorian isolates were unique.

Conclusion: These findings suggest that the nucleotide sequences flanking the bla_CTX-M genes may be useful for resolving ARG transmission pathways, especially inter-regional analyses.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.