Propionylation of Fis K32 in Salmonella enterica serovar Typhi: a key modification affecting pathogenicity.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-03-01 Epub Date: 2025-01-30 DOI:10.1080/17460913.2025.2460338

Hao Tang, Ziyang Zhan, Xiucheng Liu, Xinxiang Huang

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引用次数: 0

Abstract

Aim: This study aims to explore the role of propionylation at the K32 residue of the global regulator Fis in Salmonella enterica serovar Typhi (S. Typhi) and its influence on the pathogenicity of the bacteria.

Materials & methods: Bacterial strains were cultured in media with sodium propionate supplementation. The propionylation status of Fis was determined through Western blot and mass spectrometry analyses. The DNA-binding capability of Fis was assessed using EMSA. The invasion and survival capacities of S. Typhi were examined using T84 cells and THP-1 macrophages.

Results: Propionylation at the K32 site of Fis was found to down-regulate its DNA-binding ability, leading to a reduction in the invasion and survival of S. Typhi within host cells. The K32Q mutant exhibited significantly decreased invasion and survival capabilities compared to the wild-type and K32R mutant strains.

Conclusion: Propionylation of Fis at the K32 residue impacts the pathogenicity of S. Typhi, shedding light on the role of post-translational modifications in bacterial infections.

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目的：本研究旨在探讨全球调节因子Fis的K32残基丙酰化在伤寒沙门氏菌（S. Typhi）中的作用及其对致病性的影响。材料与方法：在添加丙酸钠的培养基中培养菌株。通过Western blot和质谱分析确定Fis的丙酰化状态。采用EMSA评价Fis的dna结合能力。采用T84细胞和THP-1巨噬细胞检测伤寒沙门氏菌的侵袭和存活能力。结果：发现Fis的K32位点丙酸化可下调其dna结合能力，导致S. Typhi在宿主细胞内的侵袭和存活减少。与野生型和K32R突变株相比，K32Q突变株的入侵和存活能力显著降低。结论：Fis在K32残基上的丙酸化影响了伤寒沙门氏菌的致病性，揭示了翻译后修饰在细菌感染中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.