Molecular xenomonitoring of Wuchereria bancrofti infection in three different evaluation settings of lymphatic filariasis elimination programme in India

IF 4.8 2区医学 Q1 INFECTIOUS DISEASES

International Journal of Infectious Diseases Pub Date : 2025-03-01 DOI:10.1016/j.ijid.2025.107807

Venkatesan Vasuki , Kaliannagounder Krishnamoorthy , Swaminathan Subramanian , Candasamy Sadanandane , Ramalingam Balasubramaniyan , Neelavathi Sivagnaname , Veerappan Padmanaban , Balakrishnan Vijayakumar , Vinayagam Sundarraj , Chokkalingam Palaniswamy , Prameela Baral , Purushothaman Jambulingam

{"title":"Molecular xenomonitoring of Wuchereria bancrofti infection in three different evaluation settings of lymphatic filariasis elimination programme in India","authors":"Venkatesan Vasuki , Kaliannagounder Krishnamoorthy , Swaminathan Subramanian , Candasamy Sadanandane , Ramalingam Balasubramaniyan , Neelavathi Sivagnaname , Veerappan Padmanaban , Balakrishnan Vijayakumar , Vinayagam Sundarraj , Chokkalingam Palaniswamy , Prameela Baral , Purushothaman Jambulingam","doi":"10.1016/j.ijid.2025.107807","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>Molecular xenomonitoring (MX) is a recommended post-validation surveillance tool for detecting early signs of lymphatic filariasis (LF) transmission. This study reports the MX results for <em>Culex quinquefasciatus</em> transmitted <em>Wuchereria bancrofti</em> in three evaluation units (EUs) in different settings of LF elimination in India.</div></div><div><h3>Methods</h3><div>Female <em>Cx. quinquefasciatus</em> mosquitoes were collected using gravid traps from 150 households in each EU. Two pools of 25 mosquitoes from each household were assayed by real-time quantitative polymerase chain reaction for <em>W. bancrofti</em> DNA and RNA. The agreement between MX and the transmission assessment survey (TAS) in the mass drug administration (MDA) stopping decision was assessed. Additionally, the microfilaria (Mf) prevalence was compared in TAS-failed EU.</div></div><div><h3>Results</h3><div>Vector infection prevalence was 0.05% and 0.07% in TAS-cleared EUs and 1.85% in TAS-failed EU. MX corroborated the TAS decision in all three settings. Mf prevalence was >1% at five sites in TAS-failed EU. Infective mosquitoes were detected in the TAS2 passed and TAS-failed EUs. The cost of MX per pool was between US $ 26.0 and 27.9 in different settings.</div></div><div><h3>Conclusions</h3><div>MX is a potential tool for evaluating different stages of post-MDA treatment in the LF elimination program. The MX detects transmission risk areas that can be confirmed by human surveys for appropriate response.</div></div>","PeriodicalId":14006,"journal":{"name":"International Journal of Infectious Diseases","volume":"152 ","pages":"Article 107807"},"PeriodicalIF":4.8000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1201971225000311","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

Molecular xenomonitoring (MX) is a recommended post-validation surveillance tool for detecting early signs of lymphatic filariasis (LF) transmission. This study reports the MX results for Culex quinquefasciatus transmitted Wuchereria bancrofti in three evaluation units (EUs) in different settings of LF elimination in India.

Methods

Female Cx. quinquefasciatus mosquitoes were collected using gravid traps from 150 households in each EU. Two pools of 25 mosquitoes from each household were assayed by real-time quantitative polymerase chain reaction for W. bancrofti DNA and RNA. The agreement between MX and the transmission assessment survey (TAS) in the mass drug administration (MDA) stopping decision was assessed. Additionally, the microfilaria (Mf) prevalence was compared in TAS-failed EU.

Results

Vector infection prevalence was 0.05% and 0.07% in TAS-cleared EUs and 1.85% in TAS-failed EU. MX corroborated the TAS decision in all three settings. Mf prevalence was >1% at five sites in TAS-failed EU. Infective mosquitoes were detected in the TAS2 passed and TAS-failed EUs. The cost of MX per pool was between US $ 26.0 and 27.9 in different settings.

Conclusions

MX is a potential tool for evaluating different stages of post-MDA treatment in the LF elimination program. The MX detects transmission risk areas that can be confirmed by human surveys for appropriate response.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Infectious Diseases 医学-传染病学

CiteScore

18.90

自引率

2.40%

发文量

1020

审稿时长

30 days

期刊介绍： International Journal of Infectious Diseases (IJID) Publisher: International Society for Infectious Diseases Publication Frequency: Monthly Type: Peer-reviewed, Open Access Scope: Publishes original clinical and laboratory-based research. Reports clinical trials, reviews, and some case reports. Focuses on epidemiology, clinical diagnosis, treatment, and control of infectious diseases. Emphasizes diseases common in under-resourced countries.