{"title":"Focus on the Holdrinet index: Toward blast quantification by flow cytometry","authors":"Edouard Bonneville, Marie-Christine Jacob, Simon Chevalier, Martine Pernollet, Chantal Dumestre-Perard, Giovanna Clavarino","doi":"10.1002/cyto.b.22225","DOIUrl":null,"url":null,"abstract":"<p>Blast quantification in the bone marrow (BM) is crucial for evaluating myeloid neoplasms, with cytomorphology being the only recognized analysis. The CD34 myeloid cell (CD34M) count by flow cytometry is promising but impaired by BM hemodilution. A modified version of the Holdrinet index (mHI) is routinely used to assess it, though not yet validated. By analyzing two differently hemodiluted BM samples from 51 patients with suspicion of myelodysplasia, this study confirms mHI accuracy in assessing BM white blood cell purity. mHI-adjusted count by flow cytometry of BM-exclusive cell subsets, such as CD34 myeloid cells, may offer a reliable and practical alternative to cytomorphology analysis, independently from hemodilution.</p>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"108 2","pages":"137-145"},"PeriodicalIF":2.3000,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.b.22225","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytometry Part B: Clinical Cytometry","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22225","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Blast quantification in the bone marrow (BM) is crucial for evaluating myeloid neoplasms, with cytomorphology being the only recognized analysis. The CD34 myeloid cell (CD34M) count by flow cytometry is promising but impaired by BM hemodilution. A modified version of the Holdrinet index (mHI) is routinely used to assess it, though not yet validated. By analyzing two differently hemodiluted BM samples from 51 patients with suspicion of myelodysplasia, this study confirms mHI accuracy in assessing BM white blood cell purity. mHI-adjusted count by flow cytometry of BM-exclusive cell subsets, such as CD34 myeloid cells, may offer a reliable and practical alternative to cytomorphology analysis, independently from hemodilution.
期刊介绍：
Cytometry Part B: Clinical Cytometry features original research reports, in-depth reviews and special issues that directly relate to and palpably impact clinical flow, mass and image-based cytometry. These may include clinical and translational investigations important in the diagnostic, prognostic and therapeutic management of patients. Thus, we welcome research papers from various disciplines related [but not limited to] hematopathologists, hematologists, immunologists and cell biologists with clinically relevant and innovative studies investigating individual-cell analytics and/or separations. In addition to the types of papers indicated above, we also welcome Letters to the Editor, describing case reports or important medical or technical topics relevant to our readership without the length and depth of a full original report.
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