{"title":"Chemical shift assignments of DRB2 domains, a dsRNA binding protein in A. thaliana RNAi pathway.","authors":"Upasana Rai, Debadutta Patra, Mandar V Deshmukh","doi":"10.1007/s12104-025-10220-x","DOIUrl":null,"url":null,"abstract":"<p><p>In Arabidopsis thaliana, micro-RNA regulation is primarily controlled by DCL1, an RNase III enzyme, and its associated proteins. DCL1, together with DRB2, governs a specific group of miRNAs that induce the inhibition of target mRNA translation. DRB2 is a multi-domain protein containing two N-terminal dsRNA binding domains (dsRBD) separated by a linker, followed by an unstructured C-terminal tail. The two dsRBDs in DRB2 are involved in recognizing the miRNA precursor and aiding DCL1 in generating 21-nucleotide-long miRNA. Our study presents a nearly complete backbone chemical shift assignment of both dsRBDs and the side-chain assignment of the first dsRBD in DRB2. The data presented here lays the groundwork for future investigations into the structural, dynamic, and functional aspects of DRB2.</p>","PeriodicalId":492,"journal":{"name":"Biomolecular NMR Assignments","volume":" ","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecular NMR Assignments","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12104-025-10220-x","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0
Abstract
In Arabidopsis thaliana, micro-RNA regulation is primarily controlled by DCL1, an RNase III enzyme, and its associated proteins. DCL1, together with DRB2, governs a specific group of miRNAs that induce the inhibition of target mRNA translation. DRB2 is a multi-domain protein containing two N-terminal dsRNA binding domains (dsRBD) separated by a linker, followed by an unstructured C-terminal tail. The two dsRBDs in DRB2 are involved in recognizing the miRNA precursor and aiding DCL1 in generating 21-nucleotide-long miRNA. Our study presents a nearly complete backbone chemical shift assignment of both dsRBDs and the side-chain assignment of the first dsRBD in DRB2. The data presented here lays the groundwork for future investigations into the structural, dynamic, and functional aspects of DRB2.
期刊介绍：
Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties.
Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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