Target gene selection for sprayable dsRNA-based biopesticide against Tetranychus urticae Koch (Acari: Tetranychidae)

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2025-01-31 DOI:10.1002/ps.8675

Yifei Wang, Yuanpeng Duan, Meibin Liu, Meifeng Ren, Yue Gao, Zhongfang Liu, Pengjiu Zhang, Lifei He, Renjun Fan, Xuguo Zhou, Jing Yang

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Abstract

BACKGROUND

Because of the excessive use of synthetic chemicals, the two-spotted spider mite, Tetranychus urticae Koch, a highly polyphagous pest, has developed comprehensive resistance to a broad spectrum of pesticides with diverse modes of action, raising severe concerns over agroecosystems and human health. To resolve this emerging issue, we initiated a project to develop double-stranded RNA (dsRNA)-based biopesticides against T. urticae, aiming for a species-specific and sustainable pest management alternative.

RESULTS

To examine the uptake of dsRNAs using the egg-soaking delivery method, we fluorescently labeled extraneous dsRNAs, and later showed that T. urticae dsRNAs can permeate through eggshell in a time-dependent manner within the first 24 h. For target gene screening, silencing of Prosbeta-1 and -5 resulted in the highest mortality (>90%) and a dark body phenotype in T. urticae. Notably, each target gene was effective in both avermectin laboratory susceptible and field resistant populations. As such, Prosbeta-5 was selected as the candidate target gene for subsequent spray-induced gene silencing (SIGS). After two rounds of spray at day 5 and day 12, SIGS led to a substantial suppression of T. urticae populations (>90%).

CONCLUSION

Our combined results suggest viable molecular targets, confirm the feasibility of SIGS against T. urticae, and lay the foundation for the development of dsRNA-based biopesticides to control this devastating pest. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Abstract Image

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基于dsrna喷雾剂防治荨叶螨的靶基因选择。

背景：由于过度使用合成化学品，荨麻疹叶螨（Tetranychus urticae Koch）这一高度多食性害虫对多种作用方式的广谱农药产生了全面抗药性，对农业生态系统和人类健康造成了严重威胁。为了解决这一新兴问题，我们启动了一个基于双链RNA （dsRNA）的生物农药的开发项目，旨在开发一种具有物种特异性和可持续性的害虫管理替代方案。结果：为了检测用鸡蛋浸泡递送法对dsRNAs的摄取，我们荧光标记了外来的dsRNAs，后来发现在最初的24小时内，T. urticae的dsRNAs可以通过蛋壳以时间依赖性的方式渗透。在靶基因筛选中，沉默probeta -1和-5导致荨麻疹的最高死亡率（约90%）和黑体表型。值得注意的是，每个靶基因对阿维菌素实验室敏感群体和田间抗性群体都有效。因此，我们选择probeta -5作为后续喷雾诱导基因沉默（SIGS）的候选靶基因。经第5天和第12天两轮喷施后，SIGS对荨麻疹种群的抑制效果显著（约90%）。结论：我们的综合结果提供了可行的分子靶点，证实了SIGS对荨麻疹的可行性，为开发基于dsrna的生物农药防治这种破坏性害虫奠定了基础。©2025作者。由John Wiley & Sons Ltd代表化学工业协会出版的《害虫管理科学》。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.