Increased NOX-dependent ROS production and proportionally enhanced antioxidant response in white adipose tissue of male rats.

IF 1.6 4区医学 Q4 ENDOCRINOLOGY & METABOLISM

Archives of Endocrinology Metabolism Pub Date : 2024-11-06 eCollection Date: 2024-01-01 DOI:10.20945/2359-4292-2024-0136

Jessica de Freitas Nascimento, Keciany Alves de Oliveira, Paula Alexandre de Freitas, Júlia de Araújo Marques Falci, Renata Prado Vasconcelos, Saulo Chaves Magalhães, Talita Mendes Farias, Maria Isabel Cardoso Alonso-Vale, Adriano Cesar Carneiro Loureiro, Denise Pires de Carvalho, Rodrigo Soares Fortunato, Ariclécio Cunha de Oliveira

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引用次数: 0

Abstract

Objective: This study aimed to investigate the redox balance in subcutaneous and retroperitoneal fat pads of male and female Wistar rats.

Materials and methods: The study analyzed the activity and gene expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase, along with the production of NADPH oxidases dependent on H₂O₂ and gene expression of NOX1, NOX2, and NOX4.

Results: The retroperitoneal fat pad in males compared with females had greater NOX2 and NOX4 expression, along with higher superoxide dismutase activity. Additionally, their subcutaneous fat pad had greater NOX4 expression and higher intracellular H₂O₂ production, together with greater expression and activity of both superoxide dismutase and catalase.

Conclusion: The white adipose tissue of male rats had greater reactive oxygen species (ROS) production compared with that of female rats, but also a proportionally greater antioxidant response. These findings are important for ongoing investigations into how sex differences may be linked to the development of metabolic diseases and the unique susceptibilities of each sex.

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来源期刊

Archives of Endocrinology Metabolism Medicine-Endocrinology, Diabetes and Metabolism

CiteScore

2.90

自引率

5.90%

发文量

107

审稿时长

7 weeks

期刊介绍： The Archives of Endocrinology and Metabolism - AE&M – is the official journal of the Brazilian Society of Endocrinology and Metabolism - SBEM, which is affiliated with the Brazilian Medical Association. Edited since 1951, the AE&M aims at publishing articles on scientific themes in the basic translational and clinical area of Endocrinology and Metabolism. The printed version AE&M is published in 6 issues/year. The full electronic issue is open access in the SciELO - Scientific Electronic Library Online e at the AE&M site: www.aem-sbem.com. From volume 59 on, the name was changed to Archives of Endocrinology and Metabolism, and it became mandatory for manuscripts to be submitted in English for the online issue. However, for the printed issue it is still optional for the articles to be sent in English or Portuguese. The journal is published six times a year, with one issue every two months.