Transcriptomics-based investigation of resistance differences to swine fever between large white pigs and min pigs

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2025-02-01 DOI:10.1016/j.virusres.2025.199536

Jia Li , Deming Ren , Xiangxu Meng , Yiyun He , Lixian Wang , Xihui Sheng , Ligang Wang

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Abstract

The genetic foundations underlying the observed disease resistance in certain indigenous pig breeds, notably the Min pigs of China, present a compelling underexplored subject of study. Exploring the mechanisms of disease resistance in these breeds could lay the groundwork for genetic improvements in pig immunity, potentially augmenting overall pig productivity. In this study, whole blood samples were collected from pre- and post- swine fever vaccinated Min and Large White pigs for transcriptome sequencing. The mRNA and lncRNA in both pig breeds were analyzed, and intra-group and inter-group comparisons were also conducted. The results indicated that a greater number of immune-related pathways such as the JAK-STAT and PI3K-AKT signaling were enriched in Min pigs. Furthermore, genes involved in inflammation and antiviral responses, including IL16, IL27, USP18, and DHX58, were upregulated in post-vaccination Min pigs compared to post-vaccination Large White pigs. This heightened immune responsiveness could contribute to the observed differences in disease resistance between Min pigs and Large White pigs.

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基于转录组学的大白猪和闽猪对猪瘟抗性差异的研究。

在某些地方猪品种中观察到的抗病性的遗传基础，特别是中国的闽猪，是一个引人注目的尚未被探索的研究课题。探索这些品种的抗病机制可以为猪免疫力的遗传改良奠定基础，从而潜在地提高猪的整体产量。在本研究中，采集了猪和大白猪接种猪瘟前后的全血样本进行转录组测序。对两个猪品种的mRNA和lncRNA进行分析，并进行组内和组间比较。结果表明，Min猪的JAK-STAT、PI3K-AKT等免疫相关信号通路丰富。此外，与接种后的大白猪相比，接种后的Min猪中参与炎症和抗病毒反应的基因，包括IL16、IL27、USP18和DHX58，表达上调。这种增强的免疫反应可能有助于观察到闽猪和大白猪之间抗病性的差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.