Blastocystis in humans and domestic animals: Risk factors assessment and potential zoonotic transmission in a periurban and rural region of Northeastern Argentina

Abstract

Blastocystis is a protist that infects both human and animal hosts worldwide. This study aimed to investigate the presence of Blastocystis in humans and domestic animals living in a periurban (PZ) and rural zone (RZ) in Northeastern Argentina and to assess their relation to socio-environmental conditions and hygiene practices as risk factors for human infection. In addition, we identified Blastocystis subtypes to evaluate the risk of zoonotic transmission. A total of 563 fecal specimens were collected from 289 humans, principally children, and 274 animals. Samples were examined by coprological examination and further analysis by real-time PCR and sequencing were performed. A semi-structured questionnaire was applied to obtain socio-environmental and hygiene practices data. The results showed an overall prevalence of 41.6 % in children and 10.2 % in animals. Non-schooled children (OR = 0.54) and children from urban area (OR = 0.55) showed a lower risk of infection. Molecular analyses revealed five subtypes (ST1, ST2, ST3, ST5, and ST7) present in humans and four subtypes (ST1, ST5, ST7 and ST10) in animals. The overlap of STs between humans and domestic animals in the same household (STs 1, 5 and 7) suggests potential zoonotic transmission, underscoring the role of infected animals as a potential risk factor for human infections. Our results can inform local health authorities to promote policies aimed at reducing transmission, emphasizing the need for direct molecular-level assessments of other common environmental sources, such as water and soil, highlighting the importance of adopting a One Health approach to better understand Blastocystis circulation.