Rhodamine-functionalized carbon dots with pH-regulated FRET efficiency for ratiometric fluorescence sensing and imaging of extremely alkaline pH

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-01-28 DOI:10.1007/s00604-024-06941-w

Jing-Yuan Zhang, Dan Wang, Jia Li, Ying Tian, Si Hui Yang

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Abstract

A ratiometric fluorescent nanoprobe (CDs-Rho), synthesized through the simple covalent amide linkage between carbon dots (CDs) and pH-sensitive rhodamine dye (Rho), was designed for the precise sensing and imaging of extremely alkaline environments. The sensing mechanism involves the opposite pH-dependent fluorescence changes in CDs and Rho, respectively, coupled with pH-regulated FRET efficiency from CDs to Rho. The nanoprobe features a wide pH response window from pH 7.0 to 12.0 with a pK_a value of 11.3 and shows high sensitivity, robust anti-interference capability, and high reversibility. Moreover, the significant shifts in emission wavelength following the pH fluctuations result in two well-separated emission signals, thus ensuring the visualization of reversible and distinct color changes (from green to red) during in vivo fluorescence imaging. This work furnished a facile protocol that contributes to the advancement of a novel method for the accurate sensing and imaging of extreme alkaline environments.

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.