3D bioprinted poly(lactic acid) scaffolds infused with curcumin-loaded nanostructured lipid carriers: a promising approach for skin regeneration

IF 5.8 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Renuka Vijayaraghavan, M. Vidyavathi, R. V. Suresh Kumar, Sravanthi Loganathan and Ravi Babu Valapa
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Abstract

Nanotechnology and 3D bioprinted scaffolds are revolutionizing the field of wound healing and skin regeneration. By facilitating proper cellular movement and providing a customizable structure that replicates the extracellular matrix, such technologies not only expedite the healing process but also ensure the seamless integration of new skin layers, enhancing tissue repair and promoting overall cell growth. This study centres on the creation and assessment of a nanostructured lipid carrier containing curcumin (CNLC), which is integrated into a 3D bioprinted PLA scaffold system. The goal is to investigate its potential as a vehicle for delivering poorly soluble curcumin for enhanced wound healing. The developed CNLC exhibited an oval morphology and average particle size of 292 nm. The entrapment efficiency (EE) was 81.37 ± 0.85%, and the drug loading capacity was 6.59 ± 1.61%. CNLC was then integrated into PLA-based 3D bioprinted scaffolds, and physicochemical analyses were conducted to evaluate their properties. Cell viability studies carried out using fibroblast cells demonstrated that the PLA/CNLC scaffolds are non-cytotoxic. In vivo experiments showed that the PLA/CNLC scaffolds exhibited complete wound contraction and closure of full-thickness wounds within a period of 21 days. The findings confirmed the scaffold's capacity as a tool for accelerating wound healing. The research emphasises the need for using biomimetic 3D printed scaffold materials and the promise of nanobiotechnology in enhancing treatment efficacy.

Abstract Image

注入姜黄素负载的纳米结构脂质载体的3D生物打印聚乳酸支架:一种有前途的皮肤再生方法。
纳米技术和3D生物打印支架正在彻底改变伤口愈合和皮肤再生领域。通过促进适当的细胞运动和提供复制细胞外基质的可定制结构,这些技术不仅加快了愈合过程,而且确保了新皮肤层的无缝整合,增强了组织修复和促进整体细胞生长。本研究的重点是创建和评估含有姜黄素(CNLC)的纳米结构脂质载体,将其集成到3D生物打印PLA支架系统中。目的是研究其作为递送难溶性姜黄素的载体的潜力,以增强伤口愈合。制备的CNLC呈椭圆形,平均粒径为292 nm。包封效率(EE)为81.37±0.85%,载药量为6.59±1.61%。然后将CNLC整合到基于pla的3D生物打印支架中,并进行物理化学分析以评估其性能。使用成纤维细胞进行的细胞活力研究表明,PLA/CNLC支架是无细胞毒性的。体内实验表明,PLA/CNLC支架在21天内伤口完全收缩,全层伤口愈合。研究结果证实了支架作为加速伤口愈合工具的能力。该研究强调了使用仿生3D打印支架材料的必要性,以及纳米生物技术在提高治疗效果方面的前景。
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来源期刊
Biomaterials Science
Biomaterials Science MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.50%
发文量
556
期刊介绍: Biomaterials Science is an international high impact journal exploring the science of biomaterials and their translation towards clinical use. Its scope encompasses new concepts in biomaterials design, studies into the interaction of biomaterials with the body, and the use of materials to answer fundamental biological questions.
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