Triggered “On/off” Luminescent Polypeptide Bowl-Shaped Nanoparticles for Selective Lighting of Tumor Cells

IF 12.1 2区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Small Pub Date : 2025-01-29 DOI:10.1002/smll.202411432
Yaning Gao, Yin Wang, Jinhui Jiang, Ping Wei, Hui Sun
{"title":"Triggered “On/off” Luminescent Polypeptide Bowl-Shaped Nanoparticles for Selective Lighting of Tumor Cells","authors":"Yaning Gao,&nbsp;Yin Wang,&nbsp;Jinhui Jiang,&nbsp;Ping Wei,&nbsp;Hui Sun","doi":"10.1002/smll.202411432","DOIUrl":null,"url":null,"abstract":"<p>Functional polymeric nanoparticles, especially those with anisotropic structures, have shown significant potential and advantages in biomedical applications including detecting, bioimaging, antimicrobial and anticancer. Herein, tetraphenylethylene (TPE) and azobenzene modified polypeptides of poly((<sub>L</sub>-glutamic acid) tetraphenylethylene-<i>stat</i>-(<sub>L</sub>-glutamic acid)) (P(GATPE<sub>9</sub>-<i>stat</i>-GA<sub>25</sub>)) and poly((<sub>L</sub>-glutamic acid) azobenzene-<i>stat</i>-(<sub>L</sub>-glutamic acid)) (P(GAAzo<sub>5</sub>-<i>stat</i>-GA<sub>29</sub>) are synthesized, which self-assemble into bowl-shaped nanoparticles (BNPs) with controlled diameter, opening size and fluorescent property individually, or by co-assembly. Due to the quenching effect of azobenzene, the fluorescence of the coassembled BNPs is completely inhibited. Upon incubated under reduction environment, the fluorescence of the BNPs is re-excited owing to the reduction or break of azo bonds. Benefiting from the high-level azo reductase in hypoxic liver cancer cells comparing to normal liver cells, the quenched BNPs exhibit pronounced fluorescence signal in human hepatoma (HepG2) cells under hypoxic condition, demonstrating the high efficiency of the reduction-responsive luminescent BNPs for selective screening of tumor cells. In addition, it is also found that a proper opening size promotes the cellular uptake of the BNPs even with size up to micron. Overall, this study provides a fresh perspective in the controlled preparation of anisotropic polymeric nanoparticles and high efficient cancer cell screening.</p>","PeriodicalId":228,"journal":{"name":"Small","volume":"21 11","pages":""},"PeriodicalIF":12.1000,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/smll.202411432","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

Functional polymeric nanoparticles, especially those with anisotropic structures, have shown significant potential and advantages in biomedical applications including detecting, bioimaging, antimicrobial and anticancer. Herein, tetraphenylethylene (TPE) and azobenzene modified polypeptides of poly((L-glutamic acid) tetraphenylethylene-stat-(L-glutamic acid)) (P(GATPE9-stat-GA25)) and poly((L-glutamic acid) azobenzene-stat-(L-glutamic acid)) (P(GAAzo5-stat-GA29) are synthesized, which self-assemble into bowl-shaped nanoparticles (BNPs) with controlled diameter, opening size and fluorescent property individually, or by co-assembly. Due to the quenching effect of azobenzene, the fluorescence of the coassembled BNPs is completely inhibited. Upon incubated under reduction environment, the fluorescence of the BNPs is re-excited owing to the reduction or break of azo bonds. Benefiting from the high-level azo reductase in hypoxic liver cancer cells comparing to normal liver cells, the quenched BNPs exhibit pronounced fluorescence signal in human hepatoma (HepG2) cells under hypoxic condition, demonstrating the high efficiency of the reduction-responsive luminescent BNPs for selective screening of tumor cells. In addition, it is also found that a proper opening size promotes the cellular uptake of the BNPs even with size up to micron. Overall, this study provides a fresh perspective in the controlled preparation of anisotropic polymeric nanoparticles and high efficient cancer cell screening.

Abstract Image

Abstract Image

触发“开/关”发光多肽碗状纳米颗粒选择性照明肿瘤细胞
功能高分子纳米粒子,特别是具有各向异性结构的高分子纳米粒子,在生物医学检测、生物成像、抗菌和抗癌等方面具有巨大的潜力和优势。本文合成了聚(l-谷氨酸)四苯基乙烯-stat-(l-谷氨酸))(P(GATPE9-stat-GA25))和聚(l-谷氨酸)偶氮苯-stat-(l-谷氨酸))(P(GAAzo5-stat-GA29)的四苯基乙烯(TPE)和偶氮苯修饰多肽,它们可以单独或共组装成碗状纳米颗粒(BNPs),其直径、开口大小和荧光性能均受控制。由于偶氮苯的猝灭作用,共组装的BNPs的荧光被完全抑制。在还原环境下孵育后,由于偶氮键的还原或断裂,BNPs的荧光被重新激发。与正常肝细胞相比,低氧肝癌细胞中的偶氮还原酶水平较高,猝灭的BNPs在人肝癌(HepG2)细胞中表现出明显的荧光信号,证明了还原响应型发光BNPs选择性筛选肿瘤细胞的高效率。此外,研究还发现,适当的开口大小可以促进BNPs的细胞摄取,即使尺寸达到微米。总之,本研究为各向异性聚合物纳米颗粒的可控制备和高效癌细胞筛选提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Small
Small 工程技术-材料科学:综合
CiteScore
17.70
自引率
3.80%
发文量
1830
审稿时长
2.1 months
期刊介绍: Small serves as an exceptional platform for both experimental and theoretical studies in fundamental and applied interdisciplinary research at the nano- and microscale. The journal offers a compelling mix of peer-reviewed Research Articles, Reviews, Perspectives, and Comments. With a remarkable 2022 Journal Impact Factor of 13.3 (Journal Citation Reports from Clarivate Analytics, 2023), Small remains among the top multidisciplinary journals, covering a wide range of topics at the interface of materials science, chemistry, physics, engineering, medicine, and biology. Small's readership includes biochemists, biologists, biomedical scientists, chemists, engineers, information technologists, materials scientists, physicists, and theoreticians alike.
文献相关原料
公司名称
产品信息
阿拉丁
sodium chloride (NaCl, AR)
阿拉丁
sodium sulfate anhydrous (AR)
阿拉丁
sodium hydrogen carbonate (NaHCO<sub>3</sub>, AR)
阿拉丁
titanium tetrachloride (AR)
阿拉丁
benzophenone (AR)
阿拉丁
4-aminobenzophenone (AR)
阿拉丁
zinc powder (AR)
阿拉丁
hydrochloric acid (HCl, AR)
阿拉丁
dimethylformamide (DMF, HPLC)
阿拉丁
N<sub>2</sub>H<sub>4</sub>·H<sub>2</sub>O
阿拉丁
hexylamine
阿拉丁
<i>p</i>-aminoazobenzene (Azo-NH<sub>2</sub>)
阿拉丁
4-dimethylaminopyridine (DMAP)
阿拉丁
<i>N</i>, <i>N</i>′-diisopropylcarbodiimideand (DIC)
阿拉丁
epichlorohydrin
阿拉丁
<sub>L</sub>-glutamic acid
阿拉丁
Triphosgene
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信