Enhanced detection of abrin: Unraveling AP lyase activity and pioneering innovative assays for advanced biosecurity applications

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-30 DOI:10.1016/j.snb.2025.137362

Tingting Liu , Lina Dong , Jing Lv , Bin Ji , Susu Xia , Jiaxin Li , Jing Wang , Jinglin Wang , Jian Zhang , Lihua Tan , Han Jin , Shan Gao , Lin Kang , Wenwen Xin

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Abstract

Abrin, a potent ribosome-inactivating protein, is a serious threat as a biological warfare agent. Developing rapid and accurate assays for detecting abrin are crucial for early prevention of related attacks. In this study, we systematically demonstrate that abrin exhibits apurinic/apyrimidinic (AP) lyase activity, with optimal enzymatic function at 63 °C and pH 4.4. Based on this discovery, we create a fluorescence signaling assay (FSA) for swift detecting abrin and further integrate it with a lateral flow assay (LFA), resulting in a novel, portable detection platform (FSA-LFA). This combined assay allows for sensitive detection of abrin at the picogram level, surpassing the limitations of traditional N-glycosidase-based assays. The FSA-LFA platform is cost-effective, highly sensitive, and amenable to field use. Furthermore, it can be readily adapted for the detection of other ribosome-inactivating proteins, offering broad potential for rapid identification of biosecurity threats.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.