Antimicrobial Effect and Cytocompatibility After Using Different Decontamination Methods on Titanium Implant Surfaces: An In Vitro Study

IF 5.3 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Clinical Oral Implants Research Pub Date : 2025-01-29 DOI:10.1111/clr.14410

Andrea Alonso-Español, Enrique Bravo, Ana Carrillo de Albornoz, María Martínez, Katharina Doll-Nikutta, Andreas Winkel, Meike Stiesch, David Herrera, Bettina Alonso, Mariano Sanz

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引用次数: 0

Abstract

Aim

To evaluate in vitro the antibacterial efficacy and cytocompatibility of different implant-decontamination methods, using both 2D and 3D peri-implant mucosa models.

Methods

Four decontamination methods [chlorhexidine (CHX), electrolytic treatment (GS), curcumin (CUR), xanthohumol (XN)] were compared in four independent experiments, three with a 2D peri-implant mucosa model on titanium surfaces and another on a 3D peri-implant mucosa model. These decontamination procedures were tested for their antibacterial effect using a multispecies biofilm model with Streptococcus oralis , Actinomyces naeslundii , Veillonella dispar , and Porphyromonas gingivalis for 24 h. Direct cytocompatibility evaluating the impact of the treatments on tissue cells as well as indirect cytocompatibility (colonization of treated implant surfaces by tissue cells) were also tested. Both outcomes were assessed by confocal laser scanning microscopy supported by neural networks.

Results

CHX demonstrated a strong alteration of cytocompatibility and antibacterial effect, but did not remove biofilm biomass. XN and CUR demonstrated antibacterial effect and biofilm removal action, as well as cytocompatibility. GS showed antibacterial capacity with a combination of areas completely clean of biofilm with others in which a non-vital biofilm remained. In the 3D peri-implant mucosa model, XN and CUR showed maintenance of the mucosa integrity after treatment, whereas CHX and GS displayed disruption in the mucosal layers.

Conclusions

Phytotherapeutics (CUR and XN) were the most cytocompatible substances and showed the largest antimicrobial effect. GS displayed antibiofilm activity with a localized “bubble-shaped effect” and impaired tissue cell morphology and integrity, compromising cytocompatibility, and CHX showed antimicrobial capacity, without reducing biofilm biomass and with altered cytocompatibility.

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不同净化方法对钛种植体表面的抑菌效果及细胞相容性的体外研究

目的采用二维和三维种植体周围粘膜模型，评价不同种植体去污方法的体外抗菌效果和细胞相容性。方法在4个独立实验中比较4种去污方法[氯己定（CHX）、电解处理（GS）、姜黄素（CUR）、黄腐酚（XN）]，其中3种用于钛表面的二维种植周黏膜模型，另一种用于三维种植周黏膜模型。使用多物种生物膜模型，对这些去污程序的抗菌效果进行了24小时的测试，该模型包括口腔链球菌、纳氏放线菌、异细孔菌和牙龈卟啉单胞菌。还测试了直接细胞相容性评估处理对组织细胞的影响以及间接细胞相容性（处理后的种植体表面由组织细胞定植）。两种结果均通过神经网络支持的共聚焦激光扫描显微镜进行评估。结果schx具有较强的细胞相容性和抗菌作用，但不能去除生物膜生物量。XN和CUR表现出抗菌作用和生物膜去除作用，以及细胞相容性。GS显示出抗菌能力，结合区域完全清洁的生物膜和其他非重要的生物膜。在3D种植体周围粘膜模型中，XN和CUR显示治疗后粘膜完整性维持，而CHX和GS显示粘膜层破坏。结论植物治疗剂（CUR和XN）是最具细胞相容性的物质，抗菌效果最好。GS具有局部“气泡效应”的抗菌活性，损害了组织细胞的形态和完整性，损害了细胞相容性；CHX具有抗菌能力，但不减少生物膜的生物量，但改变了细胞相容性。

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来源期刊

Clinical Oral Implants Research 医学-工程：生物医学

CiteScore

7.70

自引率

11.60%

发文量

149

审稿时长

3 months

期刊介绍： Clinical Oral Implants Research conveys scientific progress in the field of implant dentistry and its related areas to clinicians, teachers and researchers concerned with the application of this information for the benefit of patients in need of oral implants. The journal addresses itself to clinicians, general practitioners, periodontists, oral and maxillofacial surgeons and prosthodontists, as well as to teachers, academicians and scholars involved in the education of professionals and in the scientific promotion of the field of implant dentistry.