Chryseobacterium salviniae sp. nov., a bacterium isolated from the roots of the aquatic plant Salvinia minima.

Abstract

A polyphasic taxonomic study was carried out on strain T9W2-O^T, isolated from the roots of the aquatic plant Salvinia minima. This isolate is rod-shaped, forms yellow/orange pigmented colonies and produces the pigment flexirubin. Nearly complete 16S rRNA gene sequence homology related the strain to Chryseobacterium, with 98.8 and 98.5% similarity to Chryseobacterium profundimaris and Chryseobacterium takakiae, respectively. Average nucleotide identity and digital DNA-DNA hybridization with the closest phylogenetic neighbour of T9W2-O^T showed differences at the species level, further confirmed by differences in several physiological characteristics. The main fatty acids are iso C_{15 : 0}, iso C_{17:1 ω9c}, iso C_{17 : 0} 3-OH and summed feature 4 (iso-C_{15 : 0} 2-OH and/or C_{16:1 ω7c}). DNA G+C content is 37.2 mol%. MK-6 is the only menaquinone found, and phosphatidylethanolamine is the dominant polar lipid. Based on the results obtained, this bacterium is assigned to the genus Chryseobacterium as a new species with the name Chryseobacterium salviniae sp. nov., type strain T9W2-O^T (=NRRL B-65715^T =DSM 118061^T).