Determining a point of departure for skin sensitization potency and quantitative risk assessment of fragrance ingredients using the GARDskin dose-response assay.

IF 4.5 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Isabelle Lee, Andy Forreryd, Mihwa Na, Isabella Schember, Maura Lavelle, Robin Gradin, Ulrika Mattson, Henrik Johansson, Shashikiran Donthamsetty, Gregory Ladics, Anne Marie Api
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Abstract

Potency and quantitative risk assessment are essential for determining safe concentrations for the formulation of potential skin sensitizers into consumer products. Several new approach methodologies (NAMs) for skin sensitization hazard assessment have been developed, validated, and adopted in OECD test guidelines. However, work is ongoing to develop NAMs for predicting skin sensitization potency on a quantitative scale for use as a point of departure (POD) in next-generation risk assessment (NGRA). GARDskin Dose-Response (DR) is an adaptation of the validated GARDskin assay (OECD TG 442E), and the readout of the assay is a quantitative potency prediction similar to the No Expected Sensitization Induction Level (NESIL) value (µg/cm2). The goal of this study was to evaluate the performance of the GARDskin DR assay for potency prediction of fragrance ingredients. One hundred (100) fragrance ingredients from a reference database covering varied structural reactivity domains and potency were tested in GARDskin DR. Materials tested had varied protein-binding reactivity alerts, including Schiff base, Michael addition, SN2, and acylation. Potency categories were predicted with a total accuracy of 37% and an approximate accuracy (exact match or off by 1 category) of 81%. Combining predicted weak and very weak categories increased total accuracy to 53% and approximate accuracy to 98%. The mean prediction error for the NESIL and local lymph node assay (LLNA) EC3 was 3.15- and 3.36-fold, respectively. Based on the results of this study, GARDskin DR is a promising predictor of skin sensitization potency with an applicability domain covering a wide range of fragrance ingredient reaction mechanisms, increasing the confidence in using the assay to conduct NGRA, ultimately reducing the need for animal testing.

使用GARDskin剂量反应试验确定香味成分皮肤致敏效力和定量风险评估的起点。
效价和定量风险评估对于确定在消费品中配制潜在皮肤致敏剂的安全浓度至关重要。经合组织测试指南已经开发、验证并采用了几种新的皮肤致敏危害评估方法(NAMs)。然而,研究人员正在开发用于定量预测皮肤致敏效力的NAMs,以作为下一代风险评估(NGRA)的起点(POD)。GARDskin剂量反应(DR)是经过验证的GARDskin测定法(OECD TG 442E)的改进,该测定法的读数是类似于无预期致敏诱导水平(NESIL)值(µg/cm2)的定量效价预测。本研究的目的是评价GARDskin DR法在香味成分效价预测中的性能。在GARDskin dr中测试了来自参考数据库的一百(100)种香料成分,涵盖了不同的结构反应性域和效力。测试的材料具有不同的蛋白质结合反应性警报,包括希夫碱、迈克尔加成、SN2和酰化。效价分类预测的总准确度为37%,近似准确度(完全匹配或相差1个类别)为81%。结合预测的弱和非常弱的类别,总准确率提高到53%,近似准确率提高到98%。NESIL和局部淋巴结测定(LLNA) EC3的平均预测误差分别为3.15倍和3.36倍。基于本研究的结果,GARDskin DR是一个很有希望的皮肤致敏效力预测指标,其适用范围涵盖了广泛的香料成分反应机制,增加了使用该分析进行NGRA的信心,最终减少了对动物试验的需求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Altex-Alternatives To Animal Experimentation
Altex-Alternatives To Animal Experimentation MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
7.70
自引率
8.90%
发文量
89
审稿时长
2 months
期刊介绍: ALTEX publishes original articles, short communications, reviews, as well as news and comments and meeting reports. Manuscripts submitted to ALTEX are evaluated by two expert reviewers. The evaluation takes into account the scientific merit of a manuscript and its contribution to animal welfare and the 3R principle.
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