Cockroaches as Reservoirs, Vectors, and Potential Sentinels of Multidrug-Resistant Bacteria in Ugandan Communities: A Retrospective Analysis.

IF 1.1 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Global Health Epidemiology and Genomics Pub Date : 2025-01-19 eCollection Date: 2025-01-01 DOI:10.1155/ghe3/5940509
Steven Kakooza, Paul Ssajjakambwe, Rebecca Nalubega, Betty Namazi, Aisha Nantume, Geoffrey Ssentamu, Esther Nabatta, David Nalumenya, Mariam Wanyana, Damien F N Munyiirwa, Dorcus Namuyinda, Sayaka Tsuchida, Kazunari Ushida, John Baligwamunsi Kaneene
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引用次数: 0

Abstract

Background: Cockroaches could play a role in the transmission dynamics of antimicrobial-resistant bacteria (ARB) at variable interfaces in Ugandan communities, acting as both reservoirs and vectors. This study investigated the burden and diversity of ARB carried by cockroaches in human settlements in Uganda, so as to understand their role in the spread of these pathogens and their potential as sentinels in antimicrobial resistance (AMR) surveillance programs. Materials and Methods: A retrospective analysis was conducted on two unpublished studies by Makerere University students. Study one and study two sampled 58 and 110 cockroaches, respectively, from secondary schools in Kampala. Cockroach species identification was determined based on physical characteristics. Bacterial isolation and characterization were performed through microbiological analyses including standard culture methods, biochemical tests, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), disc diffusion method, and polymerase chain reaction (PCR). Results: Majority of the cockroaches (over 80%) were Periplaneta americana. Multidrug resistance (MDR) was prevalent among the isolates, with over 30% of the isolates being resistant to three or more antibiotic classes. Specifically, MDR (over 90%) was rampant in the extended spectrum beta-lactamase (ESBL)- or AmpC-producing Escherichia coli and enterococci isolates. Critical World Health Organization (WHO) priority pathogens, such as ESBL-/AmpC-producing Enterobacteriaceae, and carbapenem-resistant E. coli, were also identified. The most abundant resistance determinants (tetracycline and sulphonamide) were tetA, sul1, and sul2 for E. coli, and tetM and tetL for enterococci. Conclusion: The findings accentuate the potential role of cockroaches: (1) in transmitting multidrug-resistant bacteria at the human-animal-environment interface and (2) as sentinels in the surveillance of community-generated AMR.

乌干达社区蟑螂作为多药耐药细菌的宿主、媒介和潜在哨兵:回顾性分析。
背景:在乌干达社区的不同界面上,蟑螂可能在抗微生物耐药性细菌(ARB)的传播动力学中发挥作用,既充当宿主,又充当媒介。本研究调查了乌干达人类住区蟑螂携带ARB的负担和多样性,以了解它们在这些病原体传播中的作用以及它们在抗菌素耐药性监测计划中作为哨兵的潜力。材料与方法:回顾性分析马凯雷雷大学两项未发表的研究。研究一和研究二分别从坎帕拉的中学取样了58只和110只蟑螂。蟑螂的种类鉴定是根据其生理特征来确定的。通过微生物学分析进行细菌分离和鉴定,包括标准培养方法、生化试验、基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)、圆盘扩散法和聚合酶链反应(PCR)。结果:蜚蠊以美洲大蠊居多(80%以上)。多药耐药(MDR)在分离株中普遍存在,超过30%的分离株对三种或三种以上的抗生素耐药。具体而言,MDR(超过90%)在广谱β -内酰胺酶(ESBL)或产生ampc的大肠杆菌和肠球菌分离株中猖獗。还鉴定了世界卫生组织(WHO)的重点病原体,如产生ESBL-/ ampc的肠杆菌科和耐碳青霉烯的大肠杆菌。最丰富的耐药决定因子(四环素和磺胺)是大肠杆菌的tetA、sul1和sul2,肠球菌的tetM和tetL。结论:这些发现突出了蟑螂的潜在作用:(1)在人-动物-环境界面传播多药耐药细菌;(2)在社区产生的AMR监测中作为哨兵。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Global Health Epidemiology and Genomics
Global Health Epidemiology and Genomics PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
1.40
自引率
0.00%
发文量
10
审稿时长
20 weeks
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