Hypomethylation of the low-density lipoprotein receptor class A domain containing 4 gene in rheumatoid arthritis

IF 4.5 3区医学 Q2 IMMUNOLOGY

Clinical immunology Pub Date : 2025-01-25 DOI:10.1016/j.clim.2025.110441

Jingjing Song, Zhen Liu, Fan Yang, Ting Zhang, Zhenglun Pan

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引用次数: 0

Abstract

Background

Rheumatoid arthritis (RA) is a chronic inflammatory disease linked to epigenetic changes, particularly DNA methylation. While LDLRAD4 has been implicated in RA through GWAS, its role in RA via methylation remains unclear.

Objectives

To investigate LDLRAD4 methylation patterns in RA and evaluate its potential as a diagnostic and inflammatory biomarker.

Methods

We assessed DNA methylation at specific CpG sites within LDLRAD4 in 150 RA patients and 150 healthy controls. Clinical data, including disease duration and inflammatory markers, were collected.

Results

RA patients showed significant hypomethylation of LDLRAD4, especially in the LDLRAD4–43 and LDLRAD4–44 regions. ROC analysis yielded an AUC of 0.841, indicating strong diagnostic potential. Methylation levels correlated negatively with ESR, CRP and DAS28 in the RF+/CCP- subgroup.

Conclusions

LDLRAD4 DNA present hypomethylation in rheumatoid arthritis, and methylation levels are correlated with inflammatory indicator, possibly via TGF-β signaling. Further research is needed to explore its therapeutic potential.

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类风湿性关节炎低密度脂蛋白受体A类结构域4基因的低甲基化。

背景：类风湿关节炎（RA）是一种与表观遗传变化，特别是DNA甲基化相关的慢性炎症性疾病。虽然LDLRAD4通过GWAS与RA有关，但其通过甲基化在RA中的作用尚不清楚。目的：研究RA中LDLRAD4甲基化模式，并评估其作为诊断和炎症生物标志物的潜力。方法：我们评估了150名RA患者和150名健康对照者的LDLRAD4中特定CpG位点的DNA甲基化。收集临床数据，包括疾病持续时间和炎症标志物。结果：RA患者LDLRAD4存在显著的低甲基化，特别是在LDLRAD4-43和LDLRAD4-44区域。ROC分析AUC为0.841，具有较强的诊断潜力。在RF+/CCP-亚组中，甲基化水平与ESR、CRP和DAS28呈负相关。结论：类风湿性关节炎中LDLRAD4 DNA存在低甲基化，甲基化水平与炎症指标相关，可能通过TGF-β信号传导。需要进一步的研究来探索其治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical immunology 医学-免疫学

CiteScore

12.30

自引率

1.20%

发文量

212

审稿时长

34 days

期刊介绍： Clinical Immunology publishes original research delving into the molecular and cellular foundations of immunological diseases. Additionally, the journal includes reviews covering timely subjects in basic immunology, along with case reports and letters to the editor.