PEC-EC dual-mode biosensing platform for PFOA assay based on competitive binding between HSA and PFOA or hemin

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-27 DOI:10.1016/j.snb.2025.137326

Hejie Zheng , Linghe Bai , Cuicui Du, Xiaohua Zhang, Jinhua Chen

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引用次数: 0

Abstract

Perfluorooctanoic acid (PFOA), a typical persistent per- and poly-fluoroalkyl substance (PFAS), may accumulate in the body and cause various human health problems. Herein, a novel photoelecrochemical (PEC)-electrochemical (EC) dual-mode biosensing platform has been developed for PFOA assay based on the competitive binding between amino-modified magnetic beads (MB)@human serum albumin (HSA) (MB@HSA) and hemin or PFOA. Through co-incubation of MB@HSA with PFOA and hemin, the MB@HSA-hemin-PFOA conjugates were obtained and collected by magnetic separation and then introduced onto the surface of the ZnCdS@ZnIn₂S₄-modified ITO electrode. After that, the PEC and EC measurements were carried out to achieve sensitive and accurate dual-mode detection of PFOA, based on the hemin-induced photocurrent enhancement of the ZnCdS@ZnIn₂S₄ heterojunction and the electrochemical properties of hemin. The developed PEC-EC dual-mode biosensing platform exhibited good selectivity, reproducibility and sensitivity with linear response ranges of 10 nM – 1 μM (PEC)/5 nM – 1 μM (EC) and detection limits of 8.71 nM (PEC)/1.46 nM (EC). The magnetic-assisted PEC-EC dual-mode sensing platform provides a new analytical route for the detection of PFOA in water samples and a new reference for environmental monitoring of PFOA.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.