Association of Epstein-Barr Virus and its clinical relevance in Human Papillomavirus-negative oral squamous cell carcinoma: A cohort study from South India

IF 2.2 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-01-21 DOI:10.1016/j.archoralbio.2025.106183

Rajesha Payaradka , Pushkal Sinduvadi Ramesh , Vinay Kumar Rajendra , Hosapatna Laxminarayana Kishan Prasad , Prakash Patil , Mohana Kumar , Devanand Devegowda , Veena Shetty

{"title":"Association of Epstein-Barr Virus and its clinical relevance in Human Papillomavirus-negative oral squamous cell carcinoma: A cohort study from South India","authors":"Rajesha Payaradka , Pushkal Sinduvadi Ramesh , Vinay Kumar Rajendra , Hosapatna Laxminarayana Kishan Prasad , Prakash Patil , Mohana Kumar , Devanand Devegowda , Veena Shetty","doi":"10.1016/j.archoralbio.2025.106183","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The study assessed the prevalence and clinical implications of Epstein Barr Virus (EBV)-positive but Human Papillomavirus (HPV)-negative oral squamous cell carcinoma (OSCC) in a tertiary care hospital setting. The overall goal was to elucidate the potential impact of EBV on OSCC disease progression and prognosis.</div></div><div><h3>Design</h3><div>A total of 134 surgically resected and histopathologically confirmed OSCC tumor biopsies were collected from a tertiary care hospital. Tumor samples were analyzed for HPV and EBV using conventional and nested PCR. Clinical and demographic data, including age, sex, risk factors, tumor stage, and survival outcomes, were collected and analyzed to assess associations between EBV status and disease characteristics.</div></div><div><h3>Results</h3><div>All the tumor samples tested negative for HPV. However, EBV was detected in 74 cases (55.3 %) using nested PCR which was confirmed by sanger sequencing. EBV prevalence was higher in males (62.1 %), and the left buccal mucosa was the most affected site, accounting for 34 % of cases. Although statistically not significant, 63.5 % (n = 47) of the EBV positive subjects had a history of consuming both tobacco and alcohol. Of these 20 subjects showing recurrence, 35 % (n = 7) were EBV positive suggesting poor prognosis for EBV positive OSCC subjects.</div></div><div><h3>Conclusion</h3><div>This study highlights a significant prevalence of EBV in HPV-negative OSCC cases, suggesting a potential oncogenic role for EBV in OSCC progression, particularly in patients with established lifestyle risk factors. These findings underscore the need for further research into EBV's molecular mechanisms in OSCC and its utility as a biomarker for prognosis and therapeutic targeting. The results advocate for region-specific strategies to better understand and manage EBV-associated OSCC, offering potential pathways to improve outcomes in high-risk populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106183"},"PeriodicalIF":2.2000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of oral biology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0003996925000111","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

The study assessed the prevalence and clinical implications of Epstein Barr Virus (EBV)-positive but Human Papillomavirus (HPV)-negative oral squamous cell carcinoma (OSCC) in a tertiary care hospital setting. The overall goal was to elucidate the potential impact of EBV on OSCC disease progression and prognosis.

Design

A total of 134 surgically resected and histopathologically confirmed OSCC tumor biopsies were collected from a tertiary care hospital. Tumor samples were analyzed for HPV and EBV using conventional and nested PCR. Clinical and demographic data, including age, sex, risk factors, tumor stage, and survival outcomes, were collected and analyzed to assess associations between EBV status and disease characteristics.

Results

All the tumor samples tested negative for HPV. However, EBV was detected in 74 cases (55.3 %) using nested PCR which was confirmed by sanger sequencing. EBV prevalence was higher in males (62.1 %), and the left buccal mucosa was the most affected site, accounting for 34 % of cases. Although statistically not significant, 63.5 % (n = 47) of the EBV positive subjects had a history of consuming both tobacco and alcohol. Of these 20 subjects showing recurrence, 35 % (n = 7) were EBV positive suggesting poor prognosis for EBV positive OSCC subjects.

Conclusion

This study highlights a significant prevalence of EBV in HPV-negative OSCC cases, suggesting a potential oncogenic role for EBV in OSCC progression, particularly in patients with established lifestyle risk factors. These findings underscore the need for further research into EBV's molecular mechanisms in OSCC and its utility as a biomarker for prognosis and therapeutic targeting. The results advocate for region-specific strategies to better understand and manage EBV-associated OSCC, offering potential pathways to improve outcomes in high-risk populations.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry