Effects of black mulberry, chokeberry, and elderberry extracts on the healing of burn wounds

Abstract

Background

In the burn affected area of the skin, the progression or deepening of wounds is related to oxidative stress. Especially in the highly susceptible stasis zone, tissues survive to the extent that they can cope with oxidative stress.

Objective

This study investigated the potential of extracts (E) derived from the fruits (F) and leaves (L) of elderberry (E), chokeberry (C), and black mulberry (M), which are rich in antioxidant properties, to enhance the recovery of the stasis zone in burn wounds.

Methods

The study employed a sample size of 56 healthy rats. The comb burn model was used. The rats were administered the extracts via gastric gavage for a period of 21 days. Histological examination and biochemical analyses were conducted on biopsies taken from the stasis zone on the 3rd, 7th, and 21st days of the study. Photography was performed for macroscopic evaluations. The histological assays included the evaluation of inflammatory cell infiltration, reepithelialization, and collagenization, as well as immunohistochemical analysis of vascular endothelial growth factor (VEGF). Bioassays pertaining to the enzymatic activities of catalase, superoxide dismutase, glutathione peroxidase, and malondialdehyde (MDA) levels were performed.

Results

In macroscopic evaluation, a significant difference was found between the groups in terms of stasis area (F=3.58, p₂< 0.001). Post-hoc analyses showed that there was a significant difference between CFE-ELE, EFE-Burn, ELE-Burn and MLE-Burn groups in terms of stasis zones (p < 0.05). However, the difference between the groups according to time was not significant (F=1.36, p = 0.16). At the end of the 21-day experiment, inflammatory cell infiltration was higher in the burn group compared to the other groups, but similar to the CFE group. Re-epithelialization was similar in the burn group compared to the fruit extract groups and significantly lower compared to the leaf extract groups. Furthermore, a significant increase in collagenization and VEGF immunoreactivity was observed in all treatment groups compared to the burn group (p < 0.05). However, no significant difference was detected between the treatment groups. The treatment groups presented a notable reduction in MDA levels in comparison to the burn group (p < 0.001).

Conclusion

This study demonstrated the efficacy of fruit and leaf extracts in burn healing. Histological examination revealed that leaf extracts exhibited superior healing effects compared to fruit extracts. These results suggest that bioactive components in fruits and leaves may have different biological effects.