Behavior and oxidative stress evaluation of scorpion Tityus serrulatus (Lutz & Mello,1922) envenomation with genomic modulation and dopaminergic neutralization by antiscorpionic serum treatment

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicon Pub Date : 2025-02-01 DOI:10.1016/j.toxicon.2025.108263

Rahisa Scussel , Mírian Ívens Fagundes , Gabriel Paulino Luiz , Nathalia Coral Galvani , Fernanda F. Gava , Ellen De-Pieri , Lariani Tamires Witt Tietbohl , Taise Possamai-Della , Jorge M. Aguiar-Geraldo , Samira S. Valvassori , Vanessa Moraes de Andrade , Carlos Chávez-Olórtegui , Ricardo Andrez Machado-de-Ávila

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Abstract

Tityus serrulatus accident promote vast symptomatology related to toxins of the venom, which leads to a massive release of neurotransmitters, notably dopamine, affecting behavior and neurochemistry. The recommended treatment for envenomation is the antiscorpionic serum (SAEsc) administration. Related to this complexity of the Tityus serrulatus envenomation, this study aimed to assess organism responses to the venom, its impact on behavior, oxidative stress, neurochemistry, and genetic impacts, as well as the efficacy of SAEsc, especially concerning dopamine levels and genetic interactions. Swiss mice were divided into groups and administered different venom concentrations intracerebroventricularly to assess behavioral impacts and brain oxidative stress. Oxidative stress was evaluated through reactive oxygen species (ROS) analysis and antioxidant assays, including dichloro-dihydro-fluorescein diacetate (DCF), thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), and glutathione (GSH) measurements. Swiss mice were divided into four groups to evaluate genomic modulation, micronucleus enhancement, and dopamine levels. Additionally, SAEsc's neutralizing effect on dopamine was also investigated. Results showed that venom doses (100–300 ng/μL) increased lipid peroxidation in the brain, with SAEsc maintaining dopamine balance and neutralizing venom up to 24 h post-envenomation. After 24 h, cellular repair became less efficient, leading to mutagenic damage in both treated and untreated animals. The results highlight the importance of considering genomic and neurotransmitter function modulation in the treatment of Tityus serrulatus envenomation.

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（Lutz & Mello,1922）抗蝎子血清处理下的基因组调节和多巴胺能中和的毒蝎行为和氧化应激评价。

雪爪提鱼事故引起大量与毒液毒素相关的症状，导致大量神经递质释放，尤其是多巴胺，影响行为和神经化学。中毒的推荐治疗是抗蝎子血清（SAEsc）管理。考虑到蛇毒的复杂性，本研究旨在评估生物体对蛇毒的反应，其对行为、氧化应激、神经化学和遗传影响的影响，以及SAEsc的功效，特别是在多巴胺水平和遗传相互作用方面。瑞士小鼠被分成几组，并在脑室内注射不同浓度的毒液，以评估行为影响和大脑氧化应激。通过活性氧（ROS）分析和抗氧化分析评估氧化应激，包括二氯-二氢-双乙酸荧光素（DCF）、硫代巴比妥酸活性物质（TBARS）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）测定。瑞士小鼠被分为四组，以评估基因组调节、微核增强和多巴胺水平。此外，还研究了SAEsc对多巴胺的中和作用。结果表明，毒液剂量（100-300 ng/μL）增加了脑内脂质过氧化，SAEsc维持多巴胺平衡并中和毒液直至中毒后24小时。24小时后，细胞修复效率降低，导致治疗和未治疗动物的诱变损伤。这些结果强调了考虑基因组和神经递质功能调节在水貂中毒治疗中的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Toxicon 医学-毒理学

CiteScore

4.80

自引率

10.70%

发文量

358

审稿时长

68 days

期刊介绍： Toxicon has an open access mirror Toxicon: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. An introductory offer Toxicon: X - full waiver of the Open Access fee. Toxicon''s "aims and scope" are to publish: -articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms -papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins -molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins -clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained. -material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems. -articles on the translational application of toxins, for example as drugs and insecticides -epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged. -articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon. -review articles on problems related to toxinology. To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.