Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-01-27 DOI:10.1016/j.plantsci.2025.112397

Zhijuan Bian , Meiyao Chen , Lei Wang , Xiaosong Ma , Qing Yu , Zixin Jia , Jinghong Wu , Haibin Wei , Hongyan Liu , Yi Liu , Lijun Luo , Shunwu Yu

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引用次数: 0

Abstract

Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots. Rice overexpressing OsNF-YB12 was more sensitive to salinity and PEG osmotic stresses at seed germination stage, as well as reduced drought tolerance at seedling stage. Notably, the accumulation of free proline and photosynthetic efficiency was significantly declined in OsNF-YB12 transgenic plants following osmotic stimuli. Transcriptomic analysis of transgenic OsNF-YB12 plants indicated that OsNF-YB12 could upregulate terpene metabolism related to defense responses and the expression levels of JAZ proteins under normal conditions, while downregulating osmotic stress-related regulatory genes under osmotic stress, in comparison to the wild type. Further analysis revealed that overexpressing OsNF-YB12 promoted JA biosynthesis and inhibit seed germination. Haplotype analysis suggested that OsNF-YB12 may have been selected during the differentiation of indica and japonica rice varieties. Therefore, this research provides a potential molecular target for exploring and harnessing the haplotype diversity of OsNF-YB12 to enhance yield stability under drought stress during rice domestication and improvement.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.