The effect of a single dose of Mk-801 use on adult brain tissue after an experimental head trauma model applied in immature rats.

IF 1.7 4区 医学 Q3 CLINICAL NEUROLOGY
Neurological Research Pub Date : 2025-02-01 Epub Date: 2025-01-26 DOI:10.1080/01616412.2024.2448633
Ayşe Çiğel, Oya Sayın, Seren Gülşen Gürgen, Ataç Sönmez
{"title":"The effect of a single dose of Mk-801 use on adult brain tissue after an experimental head trauma model applied in immature rats.","authors":"Ayşe Çiğel, Oya Sayın, Seren Gülşen Gürgen, Ataç Sönmez","doi":"10.1080/01616412.2024.2448633","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Within the scope of this research, the long-term effects of experimental blunt head trauma on immature rats and MK-801 administered acutely after trauma on the brain tissue will be examined. In addition, the impact of trauma and MK-801 on Nestin and CD133, which are essential stem cells, will be evaluated by immunohistochemical and ELISA methods.</p><p><strong>Methods: </strong>In this study, the contusion trauma model was used. Sprague Dawley rats 30 7-day-old were divided into three groups: Group 1 (<i>n</i> = 10) control group, Group 2 (<i>n</i> = 10) trauma Group (head trauma applied), and Group 3 (<i>n</i> = 10) MK-801 + trauma Group. In the third group, immediately after head trauma, MK-801 (Sigma M107) dissolved in physiological saline was administered as a single dose of 1 mg/kg ip.</p><p><strong>Results: </strong>The concentration of nestin was significantly higher in the control group compared to both the trauma and trauma+drug groups (<i>p</i> < 0.001). CD133 was statistically significantly higher in the control group compared to the other two groups (<i>p</i> = 0.002). It was determined that the differences in Nestin CA1 and DG measurements resulted from the trauma and control and trauma and trauma+drug groups, and the differences in CD133 CA1 and DG measurements resulted from the trauma and control group.</p><p><strong>Conclusion: </strong>The positive effect of MK-801 on neuroprotective and neuronal proliferation was elaborated. Administration of MK-801 significantly induced nestin and CD133 concentrations in the injured tissue.</p>","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"105-114"},"PeriodicalIF":1.7000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01616412.2024.2448633","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/26 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Objective: Within the scope of this research, the long-term effects of experimental blunt head trauma on immature rats and MK-801 administered acutely after trauma on the brain tissue will be examined. In addition, the impact of trauma and MK-801 on Nestin and CD133, which are essential stem cells, will be evaluated by immunohistochemical and ELISA methods.

Methods: In this study, the contusion trauma model was used. Sprague Dawley rats 30 7-day-old were divided into three groups: Group 1 (n = 10) control group, Group 2 (n = 10) trauma Group (head trauma applied), and Group 3 (n = 10) MK-801 + trauma Group. In the third group, immediately after head trauma, MK-801 (Sigma M107) dissolved in physiological saline was administered as a single dose of 1 mg/kg ip.

Results: The concentration of nestin was significantly higher in the control group compared to both the trauma and trauma+drug groups (p < 0.001). CD133 was statistically significantly higher in the control group compared to the other two groups (p = 0.002). It was determined that the differences in Nestin CA1 and DG measurements resulted from the trauma and control and trauma and trauma+drug groups, and the differences in CD133 CA1 and DG measurements resulted from the trauma and control group.

Conclusion: The positive effect of MK-801 on neuroprotective and neuronal proliferation was elaborated. Administration of MK-801 significantly induced nestin and CD133 concentrations in the injured tissue.

求助全文
约1分钟内获得全文 求助全文
来源期刊
Neurological Research
Neurological Research 医学-临床神经学
CiteScore
3.60
自引率
0.00%
发文量
116
审稿时长
5.3 months
期刊介绍: Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信