The effect of a single dose of Mk-801 use on adult brain tissue after an experimental head trauma model applied in immature rats.

IF 1.7 4区 医学 Q3 CLINICAL NEUROLOGY
Neurological Research Pub Date : 2025-02-01 Epub Date: 2025-01-26 DOI:10.1080/01616412.2024.2448633
Ayşe Çiğel, Oya Sayın, Seren Gülşen Gürgen, Ataç Sönmez
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引用次数: 0

Abstract

Objective: Within the scope of this research, the long-term effects of experimental blunt head trauma on immature rats and MK-801 administered acutely after trauma on the brain tissue will be examined. In addition, the impact of trauma and MK-801 on Nestin and CD133, which are essential stem cells, will be evaluated by immunohistochemical and ELISA methods.

Methods: In this study, the contusion trauma model was used. Sprague Dawley rats 30 7-day-old were divided into three groups: Group 1 (n = 10) control group, Group 2 (n = 10) trauma Group (head trauma applied), and Group 3 (n = 10) MK-801 + trauma Group. In the third group, immediately after head trauma, MK-801 (Sigma M107) dissolved in physiological saline was administered as a single dose of 1 mg/kg ip.

Results: The concentration of nestin was significantly higher in the control group compared to both the trauma and trauma+drug groups (p < 0.001). CD133 was statistically significantly higher in the control group compared to the other two groups (p = 0.002). It was determined that the differences in Nestin CA1 and DG measurements resulted from the trauma and control and trauma and trauma+drug groups, and the differences in CD133 CA1 and DG measurements resulted from the trauma and control group.

Conclusion: The positive effect of MK-801 on neuroprotective and neuronal proliferation was elaborated. Administration of MK-801 significantly induced nestin and CD133 concentrations in the injured tissue.

单剂量Mk-801对未成熟大鼠实验性颅脑损伤模型后成年脑组织的影响。
目的:在本研究范围内,探讨实验性钝性头部创伤对未成熟大鼠的长期影响以及创伤后脑组织急性给药MK-801的影响。此外,创伤和MK-801对重要干细胞Nestin和CD133的影响将通过免疫组织化学和ELISA方法进行评估。方法:采用挫伤损伤模型。将30 7日龄的Sprague Dawley大鼠分为3组:1组(n = 10)对照组,2组(n = 10)创伤组(头部外伤),3组(n = 10) MK-801 +创伤组。在第三组中,头部外伤后立即给予溶解于生理盐水中的MK-801 (Sigma M107),单次剂量为1mg /kg / ip。结果:创伤组和创伤+药物组的nestin浓度均显著高于对照组(p p = 0.002)。我们确定Nestin CA1和DG的差异是外伤组和对照组以及外伤组和外伤+药物组造成的,CD133 CA1和DG的差异是外伤组和对照组造成的。结论:阐述了MK-801对神经保护和神经元增殖的积极作用。MK-801显著诱导损伤组织中巢蛋白和CD133的浓度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Neurological Research
Neurological Research 医学-临床神经学
CiteScore
3.60
自引率
0.00%
发文量
116
审稿时长
5.3 months
期刊介绍: Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.
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