Comparison of taurolidine with 4% ethylenediaminetetraacetic acid on antimicrobial lock effectiveness: An experimental study

IF 3.2 3区医学 Q2 NUTRITION & DIETETICS

Journal of Parenteral and Enteral Nutrition Pub Date : 2025-01-26 DOI:10.1002/jpen.2725

Jakub Visek PhD, Lenka Ryskova PhD, Petra Cesakova MD, Jana Stanclova MD, Marie Vajrychova PhD, Vladimir Blaha

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引用次数: 0

Abstract

Background

Antimicrobial lock therapy is recommended for preventing and treating catheter-related bloodstream infections, but different solutions have uncertain efficacy.

Methods

Two locks, 1.35% taurolidine and 4% ethylenediaminetetraacetic acid (EDTA), were tested on Staphylococcus epidermidis, Staphylococcus aureus, methicillin-resistant S. aureus, Pseudomonas aeruginosa, multidrug-resistant P. aeruginosa, vancomycin-resistant Enterococcus faecium, Klebsiella oxytoca (carbapenemase producing), K. pneumoniae (extended-spectrum β-lactamase producing), Candida albicans, and Candida glabrata. Broviac catheter segments were incubated with these organisms and then exposed to various lock solutions. Colony-forming units (CFUs) were counted after 2, 4, and 24 h of incubation.

Results

Taurolidine showed a significant decrease in CFUs after 2 h in S. aureus, S. epidermidis, methicillin-resistant S. aureus, vancomycin-resistant E. faecium, P. aeruginosa (both sensitive and multidrug-resistant strains), K. oxytoca, C. albicans, and C. glabrata. After 4 h, significant reductions were noted in S. aureus, S. epidermidis, methicillin-resistant S. aureus, P. aeruginosa, multidrug-resistant P. aeruginosa, K. pneumoniae, K. oxytoca, and C. albicans. Taurolidine was also effective after 24 h, especially against methicillin-resistant S. aureus and multidrug-resistant P. aeruginosa. Four percent EDTA acid showed a significant reduction in CFUs after 2 h in S. aureus, vancomycin-resistant E. faecium, P. aeruginosa, K. oxytoca, C. albicans, and C. glabrata. After 4 h, reductions occurred in P. aeruginosa, multidrug-resistant P. aeruginosa, K. oxytoca, and C. albicans and after 24 h in methicillin-resistant S. aureus, P. aeruginosa, and K. oxytoca.

Conclusion

Taurolidine is more effective than 4% EDTA acid in eradicating Gram-positive and Gram-negative microorganisms and fungi.

查看原文本刊更多论文

牛磺酸醚与4%乙二胺四乙酸抗菌锁阻效果的实验研究。

背景：抗菌锁疗法被推荐用于预防和治疗导管相关性血流感染，但不同的解决方案疗效不确定。方法：对表皮葡萄球菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌、多重耐药铜绿假单胞菌、耐万古霉素的屎肠球菌、产碳青霉烯酶克雷伯菌、产β-内酰胺酶的肺炎克雷伯菌、白色念珠菌、光假珠菌进行1.35%牛磺酸和4%乙二胺四乙酸（EDTA）锁相检测。导管段与这些微生物一起孵育，然后暴露于不同的锁定溶液中。孵育2、4、24 h后计数菌落形成单位（CFUs）。结果：牛磺酸定对金黄色葡萄球菌、表皮葡萄球菌、耐甲氧西林金黄色葡萄球菌、耐万古霉素屎球菌、铜绿假单胞菌（包括敏感和多重耐药菌株）、脱氧梭菌、白色念珠菌和光斑梭菌的cfu作用2 h后显著降低。4小时后，金黄色葡萄球菌、表皮葡萄球菌、耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌、耐多药铜绿假单胞菌、肺炎克雷伯菌、氧化克雷伯菌和白色念珠菌的数量显著减少。牛罗列丁对耐甲氧西林金黄色葡萄球菌和耐多药铜绿假单胞菌也有效。4%的EDTA酸在金黄色葡萄球菌、耐万古霉素的粪肠球菌、铜绿假单胞菌、氧梭菌、白色念珠菌和光秃念珠菌中2小时后cfu显著降低。4小时后，铜绿假单胞菌、耐多药铜绿假单胞菌、氧化绿假单胞菌和白色念珠菌数量减少，24小时后耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌和氧化绿假单胞菌数量减少。结论：牛磺酸对革兰氏阳性和革兰氏阴性微生物和真菌的杀灭效果优于4% EDTA酸。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Parenteral and Enteral Nutrition 医学-营养学

CiteScore

7.80

自引率

8.80%

发文量

161

审稿时长

6-12 weeks

期刊介绍： The Journal of Parenteral and Enteral Nutrition (JPEN) is the premier scientific journal of nutrition and metabolic support. It publishes original peer-reviewed studies that define the cutting edge of basic and clinical research in the field. It explores the science of optimizing the care of patients receiving enteral or IV therapies. Also included: reviews, techniques, brief reports, case reports, and abstracts.