Vimentin as a contributing factor in SARS-CoV-2-induced orchitis on postmortem testicular autopsy of COVID-19 cases: A case-control study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2024-01-10 eCollection Date: 2024-11-01 DOI:10.18502/ijrm.v22i11.17822
Soheila Akaberi-Nasrabadi, Azam Sabbaghi, Behzad M Toosi, Parsa Ghorbanifaraz, Gholam-Reza Mahmoudiasl, Abbas Aliaghaei, Hajarsadat Faghihi Hosseinabadi, Shahrokh Paktinat, Mohammad-Amin Abdollahifar
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引用次数: 0

Abstract

Background: Coronavirus disease 2019 (COVID-19) was identified in China in late December 2019 and led to a pandemic that resulted in millions of confirmed cases and deaths. The causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), uses distinct receptors and co-receptors to enter host cells. Vimentin has emerged as a potential co-receptor for SARS-CoV-2 due to the high level of vimentin expression in testis tissue.

Objective: The present study investigated the link between vimentin expression level and SARS-CoV-2-induced orchitis.

Materials and methods: In this case-control study, testis autopsy samples were collected immediately after the death of both COVID-19 cases and a control group that included individuals who died due to accidental causes. Gene expression and immunohistochemical assays were conducted to evaluate the level of vimentin expression, cell proliferation, and leukocyte infiltration.

Results: A significant expression of vimentin and infiltration of immune cells (CD68+, CD38+, and CD138+) in the testicular tissue of COVID-19 cases, along with extensive immunoglobulin G precipitation and reduced inhibin expression (p = 0.001) were observed. Additionally, gene expression analysis revealed increased expression of vimentin and decreased expression of the proliferation markers Ki67 and proliferating cell nuclear antigen, suggesting that SARS-CoV-2 may disrupt spermatogenesis through immune responses and the arrest of cell proliferation.

Conclusion: There may be a strong link between vimentin expression and COVID-19-induced orchitis. Further studies are needed to confirm these findings. Considering some limitations, vimentin can be used as a biomarker option for testicular damage following COVID-19-induced orchitis.

COVID-19病例死后睾丸尸检显示,维门汀是sars - cov -2诱导睾丸炎的一个因素:一项病例对照研究
背景:2019年12月下旬,中国发现了2019冠状病毒病(COVID-19),并引发了一场大流行,导致数百万确诊病例和死亡。病原体,严重急性呼吸综合征冠状病毒2 (SARS-CoV-2),使用不同的受体和共受体进入宿主细胞。由于Vimentin在睾丸组织中的高水平表达,Vimentin已成为SARS-CoV-2的潜在共受体。目的:探讨vimentin表达水平与sars - cov -2诱导的睾丸炎的关系。材料和方法:在本病例对照研究中,在COVID-19病例和对照组(包括因意外原因死亡的个体)死亡后立即收集睾丸尸检样本。通过基因表达和免疫组化检测来评估vimentin表达水平、细胞增殖和白细胞浸润。结果:新冠肺炎患者睾丸组织中vimentin表达显著,免疫细胞CD68+、CD38+、CD138+浸润显著,免疫球蛋白G大量沉淀,抑制素表达降低(p = 0.001)。此外,基因表达分析显示,vimentin表达升高,增殖标志物Ki67和增殖细胞核抗原表达降低,提示SARS-CoV-2可能通过免疫应答和细胞增殖阻滞破坏精子发生。结论:vimentin表达与covid -19诱导的睾丸炎可能存在密切联系。需要进一步的研究来证实这些发现。考虑到一些局限性,vimentin可以作为covid -19诱导的睾丸炎后睾丸损伤的生物标志物选择。
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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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