Evaluation of utilization of amplified blastocoel fluid DNA gel electrophoresis band intensity as an additional minimally invasive approach in embryo selection: A cross-sectional study.

IF 1.6 Q3 OBSTETRICS & GYNECOLOGY
International Journal of Reproductive Biomedicine Pub Date : 2024-01-10 eCollection Date: 2024-11-01 DOI:10.18502/ijrm.v22i11.17823
Fattaneh Khajehoseini, Zahra Noormohammadi, Poopak Eftekhari-Yazdi, Hamid Gourabi, Reza Pazhoomand, Shirzad Hosseinishenatal, Masood Bazrgar
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引用次数: 0

Abstract

Background: Embryo selection for transfer is critical in assisted reproduction. The presence of DNA in the blastocoel cavity of human blastocysts is assumed to be a consequence of common preimplantation chromosomal abnormalities.

Objective: This study examined the relationship between the amount of blastocoel fluid (BF) DNA and the band intensity of amplified BF-DNA in gel electrophoresis, considering the influence of ploidy status.

Materials and methods: This cross-sectional study categorizes blastocysts into 2 groups based on the array comparative genomic hybridization results by trophectoderm biopsy -the euploid and aneuploid group. After morphological scoring, a biopsy of BF was performed for whole genome amplification, followed by an assessment of band intensity and BF-DNA quantification. The relationship between BF-DNA levels, band intensity, and ploidy status were analyzed.

Results: The level of BF-DNA was higher in the aneuploid group compared to the euploid group, but the difference was not statistically significant (p = 0.2). We observed that the band intensity was affected by the ploidy status of the embryos, although this difference was not statistically significant (p = 0.059). Notably, electrophoresis band of all embryos with chromosomal loss was strong. No correlation was observed between embryo morphology and chromosomal ploidy (p = 0.8).

Conclusion: Our findings indicate that BF-DNA band intensity on agarose gel is not currently applicable for preimplantation embryo selection. It seems that embryos with chromosomal loss are more prone to DNA release to BF. The disrelation between embryo morphology and ploidy status represents the necessity of minimally invasive embryo screening methods based on ploidy status.

利用扩增囊胚液DNA凝胶电泳条带强度作为一种额外的微创胚胎选择方法的评估:一项横断面研究。
背景:胚胎移植的选择是辅助生殖的关键。人类胚泡胚腔中DNA的存在被认为是常见的着床前染色体异常的结果。目的:在考虑倍性状态影响的情况下,探讨囊胚液(BF) DNA含量与凝胶电泳扩增BF-DNA条带强度的关系。材料与方法:本横断面研究根据滋养外胚层活检阵列比较基因组杂交结果将囊胚分为两组:整倍体和非整倍体组。形态学评分后,对BF进行活检以进行全基因组扩增,随后评估条带强度和BF- dna定量。分析BF-DNA水平、条带强度与倍性状态的关系。结果:非整倍体组BF-DNA水平高于整倍体组,但差异无统计学意义(p = 0.2)。我们观察到条带强度受胚倍性状态的影响,但差异无统计学意义(p = 0.059)。值得注意的是,所有染色体丢失胚胎的电泳带都很强。胚胎形态与染色体倍性无相关性(p = 0.8)。结论:琼脂糖凝胶上的BF-DNA条带强度目前不适用于着床前胚胎选择。染色体丢失的胚胎似乎更容易向BF释放DNA。胚胎形态与倍性状态之间的不相关性说明了基于倍性状态的微创胚胎筛选方法的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.40
自引率
7.70%
发文量
93
审稿时长
16 weeks
期刊介绍: The International Journal of Reproductive BioMedicine (IJRM), formerly published as "Iranian Journal of Reproductive Medicine (ISSN: 1680-6433)", is an international monthly scientific journal for who treat and investigate problems of infertility and human reproductive disorders. This journal accepts Original Papers, Review Articles, Short Communications, Case Reports, Photo Clinics, and Letters to the Editor in the fields of fertility and infertility, ethical and social issues of assisted reproductive technologies, cellular and molecular biology of reproduction including the development of gametes and early embryos, assisted reproductive technologies in model system and in a clinical environment, reproductive endocrinology, andrology, epidemiology, pathology, genetics, oncology, surgery, psychology, and physiology. Emerging topics including cloning and stem cells are encouraged.
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