{"title":"Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity.","authors":"Amaylia Oehadian, Mohammad Ghozali, Sutiadi Kusuma, Lusi Mersiana, Nadia Gita Ghassani, Fransisca Fransisca, Yitzchak Millenard Sigilipu, Andini Kartikasari, Laniyati Hamijoyo","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets-classical (CD14++CD16-), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)-are associated with systemic lupus erythematosus (SLE) progression. This study aims to determine the association of monocyte subsets with SLE disease activity.</p><p><strong>Methods: </strong>A cross-sectional study involving 25 patients with SLE was conducted. Blood samples were collected, and monocyte subsets were identified using flow cytometry. Patients were grouped by disease activity using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) into inactive (SLEDAI-2K ≤ 4) and active (SLEDAI-2K > 4). The cutoff for monocyte subsets was determined using Receiver Operating Characteristic (ROC) analysis.</p><p><strong>Results: </strong>Nine active and 16 inactive subjects were identified. Compared with individuals without active disease, individuals with active disease had significantly lower mean classical monocyte subsets (71.9% vs 88%, p = 0.008), and higher median intermediate monocytes (29.1% vs 11.1%, p = 0.019). The median nonclassical monocyte subsets were not significantly different between the two groups. The cutoff for classical monocytes in active disease was ≤72.2%, AUC = 0.788, p = 0.021, with 66.7% sensitivity and 87.5% specificity; for intermediate monocytes, it was >22.3%, AUC = 0.788, p = 0.014, with 66.7% sensitivity and 100% specificity.</p><p><strong>Conclusion: </strong>Classical and intermediate monocytes could be considered as immune cellular markers for identifying active SLE.</p>","PeriodicalId":6889,"journal":{"name":"Acta medica Indonesiana","volume":"56 4","pages":"469-475"},"PeriodicalIF":0.5000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta medica Indonesiana","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets-classical (CD14++CD16-), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)-are associated with systemic lupus erythematosus (SLE) progression. This study aims to determine the association of monocyte subsets with SLE disease activity.
Methods: A cross-sectional study involving 25 patients with SLE was conducted. Blood samples were collected, and monocyte subsets were identified using flow cytometry. Patients were grouped by disease activity using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) into inactive (SLEDAI-2K ≤ 4) and active (SLEDAI-2K > 4). The cutoff for monocyte subsets was determined using Receiver Operating Characteristic (ROC) analysis.
Results: Nine active and 16 inactive subjects were identified. Compared with individuals without active disease, individuals with active disease had significantly lower mean classical monocyte subsets (71.9% vs 88%, p = 0.008), and higher median intermediate monocytes (29.1% vs 11.1%, p = 0.019). The median nonclassical monocyte subsets were not significantly different between the two groups. The cutoff for classical monocytes in active disease was ≤72.2%, AUC = 0.788, p = 0.021, with 66.7% sensitivity and 87.5% specificity; for intermediate monocytes, it was >22.3%, AUC = 0.788, p = 0.014, with 66.7% sensitivity and 100% specificity.
Conclusion: Classical and intermediate monocytes could be considered as immune cellular markers for identifying active SLE.
背景：单核细胞是进化保存的先天免疫细胞，在免疫应答调节中发挥重要作用。三种活化的单核细胞亚群-经典（CD14++CD16-），中间（CD14++CD16+）和非经典(CD14+CD16++)-与系统性红斑狼疮（SLE）进展有关。本研究旨在确定单核细胞亚群与SLE疾病活动性的关系。方法：对25例SLE患者进行横断面研究。采集血液样本，流式细胞术鉴定单核细胞亚群。采用系统性红斑狼疮疾病活动性指数2000 （SLEDAI-2K）将患者按疾病活动性分组为非活动性（SLEDAI-2K≤4）和活动性（SLEDAI-2K bbbb4）。采用受试者工作特征（ROC）分析确定单核细胞亚群的截止值。结果：确定了9名活跃受试者和16名不活跃受试者。与没有活动性疾病的个体相比，活动性疾病个体的平均经典单核细胞亚群显著降低（71.9% vs 88%, p = 0.008），中间单核细胞中位数较高（29.1% vs 11.1%, p = 0.019）。中位非经典单核细胞亚群在两组间无显著差异。活动性疾病经典单核细胞的临界值≤72.2%,AUC = 0.788, p = 0.021，敏感性66.7%，特异性87.5%；中间单核细胞为>22.3%,AUC = 0.788, p = 0.014，敏感性66.7%，特异性100%。结论：经典单核细胞和中间单核细胞可作为鉴别活动性SLE的免疫细胞标志物。
期刊介绍：
Acta Medica Indonesiana – The Indonesian Journal of Internal Medicine is an open accessed online journal and comprehensive peer-reviewed medical journal published by the Indonesian Society of Internal Medicine since 1968. Our main mission is to encourage the novel and important science in the clinical area in internal medicine. We welcome authors for original articles (research), review articles, interesting case reports, special articles, clinical practices, and medical illustrations that focus on the clinical area of internal medicine. Subjects suitable for publication include, but are not limited to the following fields of: -Allergy and immunology -Emergency medicine -Cancer and stem cells -Cardiovascular -Endocrinology and Metabolism -Gastroenterology -Gerontology -Hematology -Hepatology -Tropical and Infectious Disease -Virology -Internal medicine -Psychosomatic -Pulmonology -Rheumatology -Renal and Hypertension -Thyroid
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