A One-Step Plasma Assisted Synthesis of Gold Nanoparticles and Simultaneous Linker-Free Conjugation with Nestin: An In Vitro Study of Cellular Toxicity

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-01-27 DOI:10.1021/acs.nanolett.4c05641

Melika Sanavandi, Kimia Aalikhani, Mojtaba Shafiee, Hodjatallah Rabbani, Ghazaleh Fazli, Niloufar Sadeghi, Babak Shokri

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Abstract

We present a method for conjugating antigens to gold nanoparticles (GNPs) during their synthesis via gas plasma, eliminating the need for chemical linkers and significantly speeding up the process (taking only 15 min). This fast, linker-free method produces biocompatible and stable GNPs, with potential for immunotherapy applications, such as antigen and antibody conjugation and drug delivery. We demonstrate the conjugation of the antigen Nestin (NES), a tumor marker, to GNPs using two approaches. The first method involves synthesizing GNPs with citrate, followed by NES conjugation via plasma. The second method synthesizes and conjugates GNPs to NES simultaneously using plasma treatment. Conjugation was confirmed by Enzyme-Linked Immunosorbent Assay, Zeta-sizer, UV–vis spectroscopy, and Transmission Electron Microscopy. In addition, the toxicity of the prepared samples was investigated in vitro using peripheral blood mononuclear cells (PBMCs) and flow cytometry, which proved the nontoxicity of the samples.

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一步等离子体辅助合成金纳米粒子并同时与 Nestin 进行无链接共轭：细胞毒性的体外研究

我们提出了一种通过气体等离子体将抗原与金纳米颗粒（GNPs）结合的方法，消除了对化学连接的需要，并显著加快了这一过程（仅需15分钟）。这种快速、无连接的方法产生生物相容性和稳定的GNPs，具有免疫治疗应用的潜力，例如抗原和抗体偶联以及药物递送。我们展示了抗原巢蛋白（NES），肿瘤标志物，结合到GNPs使用两种方法。第一种方法是用柠檬酸盐合成GNPs，然后通过等离子体偶联NES。第二种方法是利用等离子体处理同时合成GNPs和NES。通过酶联免疫吸附试验，Zeta-sizer，紫外可见光谱和透射电子显微镜确认偶联。此外，利用外周血单核细胞（PBMCs）和流式细胞术检测了制备的样品的体外毒性，证实了样品的无毒性。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.