Construction of Faraday-cage-based aptasensor for detection of Staphylococcus aureus using high-conductivity zirconium/ hafnium metal-organic framework nanofilms

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-27 DOI:10.1016/j.snb.2025.137307

Yuting Zhang , Xueting Fan , Xiaoyi Wang, Ke Shi, Xiaoli Wang, Nandi Zhou

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引用次数: 0

Abstract

A Faraday-cage-based aptasensor based on two-dimensional (2D) high-conductivity zirconium/hafnium-based metal-organic framework (Zr/Hf-MOF) nanofilms is constructed for whole-cell detection of Staphylococcus aureus (S. aureus). A series of Zr-based MOF nanofilms with different doping ratios of hafnium was synthesized using a bottom-up approach, which consists of ferrocenedicarboxylic acid as the ligand and zirconium/hafnium as the central atoms. By investigating the composition, morphologies, and electrochemical properties of these Zr/Hf-MOF nanofilms, Zr-MOF doped the 5 % hafnium (Zr/0.05Hf-MOF) was selected as a 2D signal probe for constructing a Faraday-cage-based aptasensor because of its large size and optimal conductivity. When S. aureus is present, the Zr/0.05Hf-MOF nanofilm is attached to the electrode surface to form a Faraday-cage-based sensing interface, which extends the outer Helmholtz plane layer to lead to a direct electron transmission between the electrode and the signal probes. Under optimized conditions, the limit of detection for S. aureus reaches as low as 1.8 CFU·mL^–1. Tests on real samples demonstrate that the Faraday-cage-based aptasensor provides an accurate and feasible tool for the rapid detection of whole-cell S. aureus, holding great potential for applications in food safety and medical diagnostics.

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基于二维（2D）高导电性锆/铪基金属有机框架（Zr/Hf-MOF）纳米薄膜构建了一种法拉第笼式传感器，用于金黄色葡萄球菌（S. aureus）的全细胞检测。采用自下而上的方法，以二茂铁二羧酸为配体，锆/铪为中心原子，合成了一系列不同铪掺杂比的锆基 MOF 纳米薄膜。通过研究这些 Zr/Hf-MOF 纳米薄膜的组成、形态和电化学特性，掺杂了 5% 铪的 Zr-MOF （Zr/0.05Hf-MOF）因其尺寸大、导电性能最佳而被选为构建基于法拉第笼的灵敏传感器的二维信号探针。当有金黄色葡萄球菌存在时，Zr/0.05Hf-MOF 纳米薄膜附着在电极表面，形成一个基于法拉第笼的传感界面，它扩展了外层赫尔姆霍兹平面层，导致电子在电极和信号探针之间直接传输。在优化条件下，金黄色葡萄球菌的检测限低至 1.8 CFU-mL-1。对真实样品的测试表明，基于法拉第笼的灵敏传感器为快速检测全细胞金黄色葡萄球菌提供了一种准确可行的工具，在食品安全和医疗诊断领域具有巨大的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.