Vibration induced flow facilitating immunoagglutination for rapid detection and quantification of nanoparticles

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-27 DOI:10.1016/j.snb.2025.137312

Kanji Kaneko , Mamiko Tsugane , Taku Sato , Takeshi Hayakawa , Yosuke Hasegawa , Hiroaki Suzuki

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引用次数: 0

Abstract

The detection of bioactive nanoparticles (NPs) plays an important role in the medical and diagnostic fields. Conventional techniques for the sensitive detection of target NPs must overcome challenges such as long processing time, complex sample preparation, and high cost. Here, we show that vibration-induced flow (VIF), in which a local flow is induced around microscopic objects by applying small periodic vibrations, can facilitate immunoagglutination to realize rapid, facile, sensitive, and low-cost detection of NPs in a minute sample. In the proposed system, the presence of NPs in a minute sample fluid is detected by the formation of aggregates of affinity microparticles (MPs) stirred by the VIF within a short time (approximately 15 min). The concentration of NPs can be quantified using the average area of aggregates observed in bright-field microscopic images. Finally, we demonstrate the detection of extracellular vesicles (EVs) to validate the applicability of the proposed system in diagnostic applications.

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生物活性纳米粒子（NPs）的检测在医疗和诊断领域发挥着重要作用。对目标 NPs 进行灵敏检测的传统技术必须克服处理时间长、样品制备复杂和成本高等挑战。在这里，我们展示了振动诱导流（VIF），即通过施加微小的周期性振动在微观物体周围诱导局部流动，从而促进免疫凝集，实现对微小样品中 NPs 的快速、简便、灵敏和低成本检测。在所提出的系统中，微量样本液体中是否存在 NPs，可通过 VIF 在短时间内（约 15 分钟）搅拌形成的亲和微颗粒（MPs）聚集体进行检测。NPs 的浓度可通过明视野显微镜图像中观察到的聚集体平均面积进行量化。最后，我们演示了细胞外囊泡 (EV) 的检测，以验证拟议系统在诊断应用中的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.