LncRNA FENDRR/ miR-424-5p serves as a diagnostic biomarker for sepsis and its predictive value for clinical outcomes

IF 2.5 4区医学 Q3 IMMUNOLOGY

Immunobiology Pub Date : 2025-01-18 DOI:10.1016/j.imbio.2025.152870

Xue Luo , Xin Chen , Ying Gu , Honggang Jia , Xinyu Lin , Ling Wang , Jingyun Feng

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引用次数: 0

Abstract

Purpose

This study intends to investigate the relationship between FENDRR and miR-424-5p and their clinical significance in sepsis, aiming to provide new diagnostic markers and prognostic markers for sepsis.

Methods

136 patients with sepsis and 132 healthy volunteers were included as study subjects. The expression levels of FENDRR and miR-424-5p were detected by qPCR. ROC was applied to evaluate the diagnostic value of FENDRR and miR-424-5p. COX analyzed the independent risk factors for the occurrence of death in sepsis patients. Dual luciferase reporter assay detected the binding of FENDRR and miR-424-5p. The miR-424-5p target genes were predicted and enriched for GO function and KEGG pathway.

Results

FENDRR was up-regulated and miR-424-5p was down-regulated in patients with sepsis. FENDRR can target and bind to miR-424-5p. Both FENDRR and miR-424-5p showed significant diagnostic potential in sepsis and their combination significantly improved the diagnostic efficiency. FENDRR/miR-424-5p were significantly correlated with WBC, CRP, APACH II, and SOFA of sepsis patients. FENDRR and miR-424-5p were independent risk factors for mortality in sepsis patients. Sepsis patients with high FENDRR levels or low miR-424-5p levels had higher mortality. GO and KEGG enrichment analyses revealed that the targets of miR-424-5p were predominantly associated with cell functions and inflammatory signaling pathways.

Conclusion

Upregulated FENDRR and downregulated miR-424-5p expression can serve as biomarkers of sepsis with predictive value on the onset and prognostic outcome. FENDRR and miR-424-5p were correlated with the severity of sepsis and FENDRR can play a function in the sepsis progression via targeting miR-424-5p.

查看原文本刊更多论文

LncRNA FENDRR/ miR-424-5p可作为败血症的诊断性生物标志物及其对临床结果的预测价值。

目的：本研究旨在探讨FENDRR与miR-424-5p在脓毒症中的关系及其临床意义，旨在为脓毒症提供新的诊断指标和预后指标。方法：选取136例脓毒症患者和132名健康志愿者作为研究对象。采用qPCR检测FENDRR和miR-424-5p的表达水平。采用ROC法评价FENDRR和miR-424-5p的诊断价值。COX分析脓毒症患者死亡发生的独立危险因素。双荧光素酶报告基因检测检测到FENDRR与miR-424-5p的结合。miR-424-5p靶基因被预测并富集用于GO功能和KEGG通路。结果：脓毒症患者中FENDRR上调，miR-424-5p下调。FENDRR可以靶向并结合miR-424-5p。FENDRR和miR-424-5p在脓毒症中的诊断潜力均显著，两者联合使用可显著提高诊断效率。FENDRR/miR-424-5p与脓毒症患者WBC、CRP、APACH II、SOFA有显著相关性。FENDRR和miR-424-5p是脓毒症患者死亡的独立危险因素。FENDRR水平高或miR-424-5p水平低的脓毒症患者死亡率更高。GO和KEGG富集分析显示，miR-424-5p的靶标主要与细胞功能和炎症信号通路相关。结论：FENDRR表达上调和miR-424-5p表达下调可作为脓毒症的生物标志物，对脓毒症的发病和预后具有预测价值。FENDRR和miR-424-5p与脓毒症的严重程度相关，FENDRR可以通过靶向miR-424-5p在脓毒症的进展中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.