APPLICATION OF ULTRASOUND TECHNOLOGY IN THE PROCESSING OF HISTOLOGICAL MATERIAL.

Q4 Medicine
Georgian medical news Pub Date : 2024-11-01
G Burkadze, N Kikalishvili, T Muzashvili
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Abstract

Aim of study: The pathological examination is one of the longest in the list of medical tests. Most of this time is spent on preparation for the slide. In addition, part of the chemical reagents needed for processing is not safe for the environment and the personal working in the laboratory. The goal of our project was to develop a new, cost-effective and eco-friendly tissue processing technology by integrating a new hardware component - an ultrasound device - into the processing protocol.

Material and methods: 28 experimental protocols were developed to create a new tissue material processing protocol. A selected broad panel of tissues was used for each experiment. 168 autopsy tissue samples with a standard set of organs were used in the examination. Processing was carried out manually under conditions of ultrasonic treatment (UP200HT). All slides were stained with hematoxylin-eosin technology. The quality of the slides was evaluated through 5 points evaluation system by 2 pathologists.

Results: Application of ultrasound technology in the processing of histological material will: reduce processing time and number of reagents required for processing; increase the cost efficiency of processing; remove harmful component xylene from the processing protocol; better preserve tissue antigenic structure at the expense of reduced tissue retention time in reagents.

Conclusions: So, the interdisciplinary use of engineering and medicine will be of great benefit to both the medical staff and the environment, as well as to patients (they will receive better research quality and reduced waiting time for examination results).

研究目的:病理检查是医学检查中最长的检查之一。大部分时间都花在准备滑梯上。此外,加工所需的部分化学试剂对环境和实验室工作人员不安全。我们项目的目标是通过将一种新的硬件组件——超声设备——集成到处理方案中,开发一种新的、具有成本效益的、环保的组织处理技术。材料和方法:制定了28个实验方案,建立了一种新的组织材料处理方案。每次实验都选用一组广泛的组织。168个尸检组织样本和一套标准的器官被用于检查。在超声处理(UP200HT)条件下进行人工加工。所有载玻片均采用苏木精-伊红技术染色。2名病理医师采用5分制对切片质量进行评价。结果:超声技术在组织学材料加工中的应用将:减少加工时间和加工所需试剂数量;提高加工成本效率;从加工方案中去除有害成分二甲苯;以减少组织在试剂中的滞留时间为代价,更好地保存组织抗原结构。结论:因此,工程与医学的交叉应用将对医务人员和环境以及患者都有很大的好处(他们将获得更好的研究质量,减少等待检查结果的时间)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Georgian medical news
Georgian medical news Medicine-Medicine (all)
CiteScore
0.60
自引率
0.00%
发文量
207
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