Immunoblot evaluation of rubella humoral immunity in Portuguese women with negative or low-positive rubella IgG

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-01-21 DOI:10.1016/j.diagmicrobio.2025.116690

Maria Chasqueira , Sara Costa , Margarida Paixão , Margarida Albuquerque , Maria José Sousa Rego , Paulo Paixão

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Abstract

Despite its low incidence in Europe, rubella's immune status is still routinely assessed using commercial immunoassays. However, some discrepancies have been observed between these tests and the Immunoblot.

The main objective of this study is to evaluate, by a commercial immunoblot, the immune status of a female population that had previously presented negative, low-positive, or equivocal rubella IgG with a commercial immunoassay. In total, 146 serum samples were reevaluated.

The results demonstrated that 81 % and 73 % of the samples, were positive by Immunoblot (Immunoblot interpretation criterion 1 and criterion 2, respectively).

Our results confirm previous studies, showing that Immunoblot can significantly reduce the false negatives for rubella in the female population. This application by avoiding unnecessary revaccinations represents a potential gain for health. However, it is pertinent to conduct an extended multidisciplinary discussion to analyse the pros and cons of this algorithm.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.