Enhanced Electrochemiluminescence from Ruthenium-Tagged Immune Complex at Flexible Chains for Sensitive Analysis of Glutamate Decarboxylase Antibody.

IF 4.9 3区工程技术 Q1 CHEMISTRY, ANALYTICAL

Biosensors-Basel Pub Date : 2025-01-15 DOI:10.3390/bios15010047

Yuyao Zhang, Li Qian, Qian Zhang, Yu Li, Yu Liu, Dechen Jiang

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Abstract

Herein, a sensitive electrochemiluminescence (ECL) immunosensor is designed by immobilizing ruthenium-tagged immune complexes at flexible poly-ethylene-glycol (PEG) chains on the electrode surface, which offers more freedom for the collision of the ruthenium complex at the electrode during the initial ECL reaction. The electrochemical characterizations confirm the loose structure of the assembled layer with the immune complex, providing an increase in the current and the resultant enhanced ECL emissions. Comparing the sensors with the rigid structure, a 34-fold increase in the maximal ECL emission is recorded when PEG3400 is used as a linker. Using the optimized protocol, the prepared immunosensor exhibits a wide-ranging linear response to the model antibody (glutamate decarboxylase antibody) ranging from 10 pg/mL to 10 ng/mL. The detection limit is almost two orders lower than the value using the classic enzyme-linked immunosorbent assay, which offers a new design to enhance ECL emissions and the resultant analytical performance.

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本文设计了一种灵敏的电化学发光（ECL）免疫传感器，将钌标记的免疫复合物固定在电极表面柔性聚乙二醇（PEG）链上，这为钌复合物在最初的 ECL 反应期间在电极上的碰撞提供了更大的自由度。电化学表征证实了免疫复合物组装层的松散结构，增加了电流，从而增强了 ECL 发射。与刚性结构的传感器相比，当使用 PEG3400 作为连接剂时，最大 ECL 发射增加了 34 倍。使用优化方案制备的免疫传感器对模型抗体（谷氨酸脱羧酶抗体）的线性响应范围很广，从 10 pg/mL 到 10 ng/mL。检测限比使用传统酶联免疫吸附测定法的检测限低近两个数量级，这为提高 ECL 发射和由此产生的分析性能提供了一种新的设计。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.