Optimization of a Sonotrode Extraction Method and New Insight of Phenolic Composition of Fucus vesiculosus.

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2025-01-14 DOI:10.3390/md23010040

Lidia Gil-Martínez, Alejandro Santos-Mejías, José Manuel De la Torre-Ramírez, Alberto Baños, Vito Verardo, Ana M Gómez-Caravaca

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Abstract

The optimization of bioactive compound extraction from Fucus vesiculosus using ultrasound-assisted extraction (UAE) via sonotrode was investigated to maximize phenolic recovery and antioxidant activity while promoting a sustainable process. Optimal conditions (40% v/v ethanol in water, 38 min, 36% amplitude) were selected to maximize phenolic recovery while considering environmental and energy sustainability by optimizing extraction efficiency and minimizing solvent and energy usage. HPLC-ESI-QTOF-MS analysis tentatively identified 25 phenolic compounds, including sulfated phenolic acids, phlorotannins, flavonoids, and halophenols, with some reported for the first time in F. vesiculosus, underscoring the complexity of this alga's metabolome. The antioxidant activity of the optimized extract was evaluated through FRAP (143.7 µmol TE/g), DPPH (EC₅₀ 105.6 µg/mL), and TEAC (189.1 µmol Trolox/g) assays. The optimized process highlights F. vesiculosus as a valuable source of natural antioxidants, with potential applications in biotechnology, cosmetics, and food industries.

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声纳电极提取方法的优化及墨角菌酚类成分的新认识。

以超声辅助提取法提取墨角藻生物活性物质为研究对象，对超声辅助提取法提取墨角藻生物活性物质的工艺进行了优化，以最大限度地提高酚类物质的回收率和抗氧化活性，同时促进工艺的可持续性。最佳条件（40% v/v乙醇在水中，38 min，振幅36%）通过优化提取效率，最大限度地减少溶剂和能源的使用，最大限度地提高酚的回收率，同时考虑环境和能源的可持续性。HPLC-ESI-QTOF-MS分析初步鉴定出25种酚类化合物，包括硫酸酸化酚酸、绿藻单宁、黄酮类化合物和盐酚类化合物，其中一些是首次在F. vesiculosus中报道，强调了该藻类代谢组的复杂性。通过FRAP（143.7µmol TE/g）、DPPH （EC50 105.6µg/mL）和TEAC（189.1µmol Trolox/g）测定优化后提取物的抗氧化活性。优化后的工艺突出了水疱酵母作为一种有价值的天然抗氧化剂来源，在生物技术、化妆品和食品工业中具有潜在的应用前景。

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.