Mohammad Neshatpour Esfahani, Majid Gholami-Ahangaran, William J. Sullivan Jr.
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引用次数: 0
Abstract
Background
This study seeks to close this divide by assessing the occurrence of Toxoplasma gondii (T. gondii) in the brain tissues of pet birds displaying neurological symptoms, utilizing Nested Polymerase Chain Reaction (PCR) and Loop-mediated Isothermal Amplification (LAMP) methods. Furthermore, it aims to evaluate and contrast the sensitivity and specificity of different diagnostic procedures. Seventy bird samples, consisting of 50 sick and 20 asymptomatic samples, were assessed using various methods. Brain tissue DNA was isolated and then analyzed using nested PCR and LAMP assays targeting the T. gondii B1 gene and RE gene. Sensitivity and specificity assessments were conducted, and statistical evaluations were made using SPSS software.
Results
Our findings revealed that no T. gondii cysts were detected in the smear preparations. However, T. gondii DNA was found in 6% of the sick birds using nested PCR, and LAMP identified T. gondii DNA in 8% of these individuals. Both techniques demonstrated a high level of specificity, with LAMP showing higher sensitivity and faster processing times than nested PCR. These results significantly contribute to our understanding of toxoplasmosis and highlight the reliability and potential for the widespread use of these diagnostic techniques, providing reassurance and confidence in their effectiveness.
Conclusion
The study underscores the importance of molecular diagnostics in promptly identifying and treating toxoplasmosis in birds. This enhances our understanding and control of the disease and underscores the significant impact of this research. The potential for further studies using more extensive and diverse bird populations to enhance surveillance and prevention strategies against T. gondii infections is promising. It should be actively pursued, giving us hope for a better future in avian health.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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