Approaches of Inducing Tolerance to Murine Schistosomiasis mansoni applying Biomphalaria and Bulinus Proteins

IF 1.2 3区 农林科学 Q4 PARASITOLOGY
Hanan S. Mossalem, Sami Mohamed Nasr, Azza Moustafa Fahmy, Shimaa Atta, Gehan Labib Abuelenain
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Abstract

Background

The freshwater snails Biomphalaria alexandrina and Bulinus trancatus are key contributors to the transmission of S. mansoni and S.haematobium, respectively, for being their intermediate hosts.

Objectives

This research study aimed to investigate the potency of the nucleoproteins (NPs) extracted from both snail species on the host immune reactions as an approach to developing a potential vaccine.

Methods

Three groups of six-week-old Swiss-Webster mice (n = 18; 15–20 g each) were injected intraperitoneally for three consecutive weeks with single doses (once a week) of B. alexandrina, B. truncatus, or a mixture of their nucleoproteins (50 µg each). On day 21st, the nucleoprotein-treated mice altogether, with six more mice, received subcutaneously S. mansoni cercariae (60/mouse). Eight weeks later, the experimental mice were sacrificed for evaluation of certain parasitological, molecular and immunological responses.

Results

The data of mice immunized with the various types of nucleoproteins showed a significant increase of FAS/R gene expressions in hepatic tissues and anti-IgG antibody levels in sera on the one hand and a significant decrease of worm loads and β-actin/R gene expression levels on the other hand when compared to the infected control mice.

Conclusion

These findings highlight the role of snails in immunomodulation and shed light on the possibility of antagonizing effects that might occur when the nucleoproteins of different species are mixed. Moreover, this research study might promote the literature spotting the importance of snail proteins against schistosomiasis.

生物phalaria蛋白和Bulinus蛋白诱导小鼠曼氏血吸虫病耐受的研究。
背景:淡水蜗牛Biomphalaria alexandrina和淡水蜗牛Bulinus trancatus作为mansoni和haematobium的中间宿主,分别是其传播的主要贡献者。目的:本研究旨在探讨从两种蜗牛中提取的核蛋白(NPs)对宿主免疫反应的效力,作为开发潜在疫苗的途径。方法:三组6周龄Swiss-Webster小鼠(n = 18;15- 20g /只)腹腔注射单剂量(每周1次)亚历山大白僵菌、truncatus白僵菌或其核蛋白的混合物(各50µg),连续3周。第21天,所有核蛋白处理小鼠和另外6只小鼠皮下注射曼氏尾蚴(60只/只)。8周后,处死实验小鼠,评估某些寄生虫学、分子和免疫学反应。结果:各类型核蛋白免疫小鼠的数据显示,与感染对照小鼠相比,肝脏组织中FAS/R基因表达和血清中抗igg抗体水平显著升高,蠕虫载量和β-肌动蛋白/R基因表达水平显著降低。结论:这些发现突出了蜗牛在免疫调节中的作用,并揭示了不同物种核蛋白混合时可能发生拮抗作用的可能性。此外,本研究可能会促进文献发现蜗牛蛋白对血吸虫病的重要性。
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来源期刊
Acta Parasitologica
Acta Parasitologica 医学-寄生虫学
CiteScore
3.10
自引率
6.70%
发文量
149
审稿时长
6-12 weeks
期刊介绍: Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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