Construction of Yarrowia lipolytica for degradation of low-density polyethylene

IF 6.9 2区环境科学与生态学 Q1 ENGINEERING, CHEMICAL

Process Safety and Environmental Protection Pub Date : 2025-01-20 DOI:10.1016/j.psep.2025.106818

Fei Liu, Ni Zhang, Yutong Shang, Mingdong Yao, Mingzhu Ding, Yingjin Yuan

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引用次数: 0

Abstract

The current extensive production and widespread use of polyethylene (PE) has resulted in the accumulation of its waste in the environment, posing a significant threat to the global ecosystem and human health. Biodegradation is regarded as an environmentally sustainable approach for plastic waste treatment. However, achieving high biodegradation efficiency of PE is primary challenge. In this study, the engineered Yarrowia lipolytica strains, expressing heterologous PE-degrading enzymes, were successfully constructed for enhancing degradation of pretreated low-density polyethylene (LDPE) at room temperature. SEM results showed significant changes in surface roughness and erosion of pretreated samples after incubation with the engineered strains of alkane hydroxylase-secreting YPE04 and laccase-secreting YPE10. Notably, YPE04 and YPE10 reduced the weight of LDPE films by 8.9 ± 0.9 % and 11.8 ± 0.7 %, respectively, within 7 days. It was further clarified by GC-MS that the main degradation products of LDPE films by the aforementioned two engineered strains were 9-octadecenoic acid and n-dodecyl methacrylate, respectively. Overall, two strains, YPE04 and YPE10, were obtained with great potential for LDPE degradation. This study not only provides a reference for the construction of efficient PE-degrading strains but also offers new ideas for the application of bioremediation technology in plastic waste management.

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来源期刊

Process Safety and Environmental Protection 环境科学-工程：化工

CiteScore

11.40

自引率

15.40%

发文量

929

审稿时长

8.0 months

期刊介绍： The Process Safety and Environmental Protection (PSEP) journal is a leading international publication that focuses on the publication of high-quality, original research papers in the field of engineering, specifically those related to the safety of industrial processes and environmental protection. The journal encourages submissions that present new developments in safety and environmental aspects, particularly those that show how research findings can be applied in process engineering design and practice. PSEP is particularly interested in research that brings fresh perspectives to established engineering principles, identifies unsolved problems, or suggests directions for future research. The journal also values contributions that push the boundaries of traditional engineering and welcomes multidisciplinary papers. PSEP's articles are abstracted and indexed by a range of databases and services, which helps to ensure that the journal's research is accessible and recognized in the academic and professional communities. These databases include ANTE, Chemical Abstracts, Chemical Hazards in Industry, Current Contents, Elsevier Engineering Information database, Pascal Francis, Web of Science, Scopus, Engineering Information Database EnCompass LIT (Elsevier), and INSPEC. This wide coverage facilitates the dissemination of the journal's content to a global audience interested in process safety and environmental engineering.