Review of advancement in aggregation-induced emission-based fluorescent biosensors for enzyme detection: Mechanisms and biomedical applications

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-01-25 DOI:10.1016/j.aca.2025.343716

Huihui Sun, Jiake Wen, Shujing Chen, Yuli Han, Omachi Daniel Ogaji, Abdulmumin Muhammad Biu, Huan Cui, Xue Meng, Jin Li, Kunze Du, Yanxu Chang

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Abstract

Background

Enzymes, primarily proteins produced by living organisms, exhibit high substrate selectivity and catalytic efficiency. Many are crucial for normal biological processes and are closely associated with the onset of various diseases. As such, developing methods for detecting disease-related enzymes is essential. Biosensors based on aggregation-induced emission (AIE) have gained significant attention due to their outstanding properties, including excellent photostability, high luminescence efficiency in the aggregated state, large Stokes shift, and favorable biocompatibility. This has led researchers to design a variety of fluorogens with AIE characteristics (AIEgens).

Results

This review provides an overview of the luminescence mechanism behind AIE and the key properties of AIEgens. It focuses on the physiological roles of disease-related enzymes and outlines various AIE-based fluorescent biosensors developed for enzyme recognition and detection. These biosensors are categorized according to their mechanisms, including hydrolysis, electrostatic adsorption, biological redox reactions, and pH-response. Additionally, this review explores the application of enzymes in disease progression, highlighting their value in inhibitor screening, traditional Chinese medicine research, sensing, bioimaging, and disease diagnosis and therapy. It also discusses the current limitations of AIEgens and explores emerging opportunities for their application.

Significance and Novelty

Enzyme activity and levels are closely linked to the development of specific diseases, underscoring the importance of advancing methods for enzyme detection in disease diagnosis and treatment. This review provides valuable insights for the development of innovative AIEgens for enzyme detection, expands the options for detection mechanisms, and offers a theoretical foundation for clinical diagnostics and therapeutic applications.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.