Development of a bioavailability-based acute effects assessment method for nickel.

Abstract

This study aimed to develop a bioavailability-based effects assessment method for nickel (Ni) to derive acute freshwater environmental thresholds in Europe. The authors established a reliable acute freshwater Ni ecotoxicity database covering 63 different freshwater species, and the existing acute Ni bioavailability models for invertebrates were revised. A single average invertebrate bioavailability model was proposed, in which the protective effects of Ca2+ and Mg2+ on Ni2+ toxicity were integrated as a single-site competition effect at the Ni biotic ligand. The biotic ligand stability constants for Ca2+ and Mg2+ (log KCaBL = 3.80 and log KMgBL = 3.32) were derived by averaging these parameters from three existing cladoceran models. A pH extension was also integrated into the average invertebrate bioavailability model to reflect the increase in free Ni2+ toxicity observed greater than pH 8.0. The proposed invertebrate model has further been validated using an extensive dataset of acute toxicity data covering 15 different invertebrate species. Evaluating the extrapolation of the invertebrate model to plant species revealed significant uncertainty about the applicability of the acute Ni bioavailability models for plants. The newly developed acute invertebrate model was used alongside the existing acute fish and algae bioavailability models to support an acute bioavailability normalization approach for Ni. By combining these bioavailability models with the acute toxicity dataset for Ni, a normalized species sensitivity distribution approach is proposed to derive site-specific acute environmental thresholds, expressed by the HC5L(E)C50 (i.e., dissolved Ni concentration resulting in at least 50% effect for 5% of the species). The applicability ranges of the acute Ni bioavailability normalization approach are estimated to be valid for approximately 70% of European freshwaters. The proposed approach serves as a basis to incorporate bioavailability into the compliance evaluation relative to acute environmental threshold values for Ni in Europe.