Masquelet technique combined with concentrated growth factors for the reconstruction of rabbit mandibular marginal bone defect.

Abstract

Objective: Both the Masquelet technique (MT) and concentrated growth factors (CGF) reduce early graft loss and improve bone regeneration. This study aims to explore the efficacy of combining MT with CGF for mandibular defect repair by characterizing the induced membrane and assessing in vivo osteogenesis.

Materials and methods: Three experimental groups were compared: negative control (NC), MT, and Masquelet combined with CGF (MTC). Four weeks after the first surgery, histopathology is used to identify the morphological structure of the induced membrane, evaluate the degree of vascularization, and the secretion levels of osteogenesis and angiogenesis-related growth factors. In vivo osteogenesis was assessed with a second autologous bone graft surgery 4 weeks later, and bone reconstruction was evaluated by micro-CT and histopathology.

Results: CGF significantly increased the induced membrane thickness, vascularization, and growth factor secretion levels. Quantitative micro-CT analysis showed that the bone volume fraction (BV/TV) at 4 weeks post-surgery was higher in the MTC group (23.30 ± 1.15%) compared to the MT group (16.50 ± 1.29%) and NC group (12.62 ± 1.23%) (P < 0.05). By 12 weeks, the difference in BV/TV between MTC (32.59 ± 0.11%) and MT (29.89 ± 0.49%) reduced, indicating consistent bone regeneration. Trabecular parameters were consistently higher in the MTC group, highlighting enhanced osteogenesis.

Conclusion: Combining the Masquelet technique with CGF effectively reduces early bone graft absorption and promotes bone repair. These findings suggest potential benefits for oral and maxillofacial bone defect treatment, though further studies are needed to confirm long-term efficacy.

Clinical relevance: Integrating Masquelet technique and CGF in mandibular reconstruction may improve clinical outcomes by enhancing bone regeneration and reducing graft failure.