Preparation and evaluation of Magnetospirillum gryphiswaldense MSR-1 bioinoculant on the growth and productivity of Vigna radiata (L.) R. Wilczek.

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-02-01 Epub Date: 2025-01-21 DOI:10.1007/s13205-025-04206-8

Shraddha Shirsat, K Suthindhiran

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引用次数: 0

Abstract

Recently, there has been a growing interest in the application of beneficial microorganisms to enhance crop performance. Magnetospirillum gryphiswaldense (MSR-1) are spiral-shaped, gram-negative bacteria that exhibit magnetotaxis with the help of magnetosomes (iron oxide or iron sulphide). They have exhibited biomedical and environmental applications; however, the agricultural utilization of these strains is yet to be explored. This study investigates the nitrogen fixation, iron supplementation, and plant growth promotion (PGP) activity of MSR-1 on Vigna radiata (Green gram). Firstly, agriculturally important genes including nif (360 bp), fur (480 bp), and feoB (675 bp) were identified in MSR-1. Further, the strain was used to produce the Magnetospirillum Liquid Biofertilizer (MLB) and analyzed using GC-MS and LC-HRMS to identify beneficial compounds for agriculture. The findings indicated the presence of biostimulant compounds including proline, indole-3-acetic acid, koninginin, and glutamate. Furthermore, different dilutions (20%, 40%, 60%, 80%, and 100%) of MLB were supplemented to V.radiata to explore its nitrogen-fixing capacity, iron enhancement effects, plant growth and yield. Plants treated with 20% MLB exhibited a significant increase in plant length (85.6%), seed weight (74.5%), total chlorophyll content (58.3%), protein (95.91%), leaf nitrogen content (71.72%) compared to the control group treated with distilled water. Under iron deficiency conditions, 60% MLB improved the plant length (70.38%), seed weight (66.6%), total chlorophyll (84.16%), protein (66.19%), iron content of leaves (80.79%) compared to the control group. The findings indicate that MSR-1 enhances plant productivity and it can be used as a source of iron fertilizer for addressing iron deficiency in plants.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04206-8.

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致密磁螺旋藻MSR-1生物接种剂的制备及对辐射弧菌生长和产量的影响r . Wilczek。

近年来，人们对利用有益微生物提高作物生产性能的兴趣日益浓厚。gryphiswaldense （MSR-1）是螺旋形的革兰氏阴性细菌，在磁小体（氧化铁或硫化铁）的帮助下表现出趋磁性。它们展示了生物医学和环境应用；然而，这些菌株的农业利用还有待探索。本研究研究了MSR-1在绿叶豆（Vigna radiata, Green gram）上的固氮、补铁和植物生长促进（PGP）活性。首先，在MSR-1中发现了nif （360 bp）、fur （480 bp）和feoB （675 bp）等重要的农业基因。利用该菌株制备了磁螺旋藻液体生物肥料（MLB），并利用GC-MS和LC-HRMS对其进行了分析，鉴定出对农业有益的化合物。研究结果表明，生物刺激素化合物包括脯氨酸、吲哚-3-乙酸、丁香素和谷氨酸。此外，在V.radiata中添加不同稀释度（20%、40%、60%、80%和100%）的MLB，考察其固氮能力、补铁效果、植株生长和产量。与蒸馏水处理的对照组相比，20% MLB处理的植株的株长（85.6%）、种子重（74.5%）、总叶绿素含量（58.3%）、蛋白质含量（95.91%）和叶片氮含量（71.72%）显著增加。在缺铁条件下，60% MLB比对照组提高了株长（70.38%）、种子重（66.6%）、总叶绿素（84.16%）、蛋白质（66.19%）和叶片铁含量（80.79%）。研究结果表明，MSR-1可以提高植物的生产力，并可作为铁肥的来源，解决植物缺铁问题。补充信息：在线版本包含补充资料，下载地址为10.1007/s13205-025-04206-8。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.