Variations in ciliary beat frequency based on chronic rhinosinusitis endotype and phenotype.

IF 5.8 2区医学 Q1 ALLERGY

Annals of Allergy Asthma & Immunology Pub Date : 2025-01-20 DOI:10.1016/j.anai.2025.01.009

Asher C Park, Theodore V Nguyen, Lidek Chou, Katelyn K Dilley, Zhikai Zhu, Akarsh Lal, Pranav Nair, Zhongping Chen, Edward C Kuan, Brian J F Wong

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引用次数: 0

Abstract

Background: Chronic rhinosinusitis (CRS) is traditionally classified into CRS with or without nasal polyps and, more recently, into eosinophilic and noneosinophilic endotypes. Limited research exists on the relationship between CRS subtypes and mucociliary function. This study compares ciliary beat frequency (CBF) across CRS subtypes.

Objective: To investigate CBF across different CRS subtypes and validate spectrally encoded interferometric microscopy (SEIM) against phase-contrast microscopy (PCM) for measuring CBF.

Methods: Sinonasal mucosa from endoscopic endonasal surgery cases were imaged ex vivo at physiological temperatures using PCM and SEIM. CBF measurements were compared between disease states (control vs CRS with nasal polyps [CRSwNPs] vs CRS without nasal polyps [CRSsNPs] and control vs eosinophilic CRS vs noneosinophilic CRS) and between PCM and SEIM.

Results: CRSwNP mucosa (5.77 ± 0.12 Hz) had significantly lower CBF compared with control (6.23 ± 0.11 Hz) (P = .001). Both eosinophilic rhinosinusitis (5.74 ± 0.16 Hz; P = .005) and noneosinophilic CRS mucosa (6.00 ± 0.08 Hz; P = .03) had significantly lower CBF compared with control (6.28 ± 0.11 Hz). There was no significant difference between PCM (7.65 ± 0.60 Hz) and SEIM (7.64 ± 0.51 Hz) as a means of evaluating CBF (P = .36).

Conclusion: Among the CRS subtypes, eosinophilic, noneosinophilic, and CRSwNP are associated with lower CBF compared with healthy controls. SEIM may have value in measuring CBF.

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来源期刊

Annals of Allergy Asthma & Immunology 医学-过敏

CiteScore

6.50

自引率

6.80%

发文量

437

审稿时长

33 days

期刊介绍： Annals of Allergy, Asthma & Immunology is a scholarly medical journal published monthly by the American College of Allergy, Asthma & Immunology. The purpose of Annals is to serve as an objective evidence-based forum for the allergy/immunology specialist to keep up to date on current clinical science (both research and practice-based) in the fields of allergy, asthma, and immunology. The emphasis of the journal will be to provide clinical and research information that is readily applicable to both the clinician and the researcher. Each issue of the Annals shall also provide opportunities to participate in accredited continuing medical education activities to enhance overall clinical proficiency.