Variations in ciliary beat frequency based on chronic rhinosinusitis endotype and phenotype

IF 5.8 2区医学 Q1 ALLERGY

Annals of Allergy Asthma & Immunology Pub Date : 2025-04-01 DOI:10.1016/j.anai.2025.01.009

Asher C. Park BS , Theodore V. Nguyen BS , Lidek Chou BS , Katelyn K. Dilley BS , Zhikai Zhu MS , Akarsh Lal BS , Pranav Nair BS , Zhongping Chen PhD , Edward C. Kuan MD, MBA , Brian J.F. Wong MD, PhD

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引用次数: 0

Abstract

Background

Chronic rhinosinusitis (CRS) is traditionally classified into CRS with or without nasal polyps and, more recently, into eosinophilic and noneosinophilic endotypes. Limited research exists on the relationship between CRS subtypes and mucociliary function. This study compares ciliary beat frequency (CBF) across CRS subtypes.

Objective

To investigate CBF across different CRS subtypes and validate spectrally encoded interferometric microscopy (SEIM) against phase-contrast microscopy (PCM) for measuring CBF.

Methods

Sinonasal mucosa from endoscopic endonasal surgery cases were imaged ex vivo at physiological temperatures using PCM and SEIM. CBF measurements were compared between disease states (control vs CRS with nasal polyps [CRSwNPs] vs CRS without nasal polyps [CRSsNPs] and control vs eosinophilic CRS vs noneosinophilic CRS) and between PCM and SEIM.

Results

CRSwNP mucosa (5.77 ± 0.12 Hz) had significantly lower CBF compared with control (6.23 ± 0.11 Hz) (P = .001). Both eosinophilic rhinosinusitis (5.74 ± 0.16 Hz; P = .005) and noneosinophilic CRS mucosa (6.00 ± 0.08 Hz; P = .03) had significantly lower CBF compared with control (6.28 ± 0.11 Hz). There was no significant difference between PCM (7.65 ± 0.60 Hz) and SEIM (7.64 ± 0.51 Hz) as a means of evaluating CBF (P = .36).

Conclusion

Among the CRS subtypes, eosinophilic, noneosinophilic, and CRSwNP are associated with lower CBF compared with healthy controls. SEIM may have value in measuring CBF.

查看原文本刊更多论文

基于慢性鼻窦炎内型和表型的纤毛搏动频率变化。

背景：慢性鼻窦炎（CRS）传统上分为伴有或不伴有鼻息肉的CRS，最近又分为嗜酸性粒细胞和非嗜酸性粒细胞内型。CRS亚型与纤毛粘膜功能的关系研究有限。本研究比较了不同CRS亚型的纤毛搏动频率（CBF）。目的：研究不同CRS亚型的脑血流，并验证光谱编码干涉显微镜（SEIM）和相差显微镜（PCM）测量脑血流的有效性。方法：应用PCM和SEIM对鼻内镜手术患者鼻黏膜进行体外生理温度成像。将CBF测量值在疾病状态（对照组与合并鼻息肉的慢性鼻窦炎（CRSwNP）与无鼻息肉的慢性鼻窦炎（CRSsNP）、对照组与嗜酸性粒细胞性慢性鼻窦炎与非嗜酸性粒细胞性慢性鼻窦炎）以及PCM和SEIM之间进行比较。结果：CRSwNP粘膜组（5.77±0.12 Hz） CBF明显低于对照组(6.23±0.11 Hz；p = 0.001)。两种嗜酸性鼻窦炎(5.74±0.16 Hz；p=0.005)和非嗜酸性CRS粘膜(6.00±0.08 Hz；p=0.03)，纤毛搏动频率明显低于对照组（6.28±0.11 Hz）。PCM（7.65±0.60 Hz）与SEIM（7.64±0.51 Hz）作为CBF评估手段无显著差异（p=0.36）。结论：与健康对照相比，在CRS亚型中，嗜酸性粒细胞、非嗜酸性粒细胞和CRSwNP与较低的纤毛搏动频率相关。SEIM在测量纤毛搏动频率方面可能有价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Allergy Asthma & Immunology 医学-过敏

CiteScore

6.50

自引率

6.80%

发文量

437

审稿时长

33 days

期刊介绍： Annals of Allergy, Asthma & Immunology is a scholarly medical journal published monthly by the American College of Allergy, Asthma & Immunology. The purpose of Annals is to serve as an objective evidence-based forum for the allergy/immunology specialist to keep up to date on current clinical science (both research and practice-based) in the fields of allergy, asthma, and immunology. The emphasis of the journal will be to provide clinical and research information that is readily applicable to both the clinician and the researcher. Each issue of the Annals shall also provide opportunities to participate in accredited continuing medical education activities to enhance overall clinical proficiency.