In Silico Prediction of Maize microRNA as a Xanthine Oxidase Inhibitor: A New Approach to Treating Hyperuricemia Patients.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2025-01-15 DOI:10.3390/ncrna11010006

Manas Joshi, Mohd Mabood Khan

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引用次数: 0

Abstract

Introduction: Hyperuricemia is characterized by increased uric acid (UA) in the body. The ability to block xanthine oxidase (XO) is a useful way to check how different bioactive molecules affect hyperuricemia. Previous reports showed the significant effect of corn against hyperuricemia disorder with its anti-XO activity. The identification of stable Zea mays miRNA (zma-miR) in humans has opened up a new avenue for speculation about its part in regulating novel human gene targets.

Aims: The aim of this study was to investigate the prospects of zma-miRs in XO gene regulation, the possible mechanism, and the interaction analysis of the zma-miR-XO mRNA transcript.

Method: Significant features of miRNA-mRNA interaction were revealed using two popular miRNA target prediction software-intaRNA (version 3.3.1) and RNA hybrid (version 2.2.1) Results: Only 12 zma-miR-156 variants, out of the 325 zma-miR's sequences reported in the miRNA database, efficiently interact with the 3'UTR of the XO gene. Characteristics of miRNA-mRNA interaction were as follows: the positioning of zma-miR-156 variants shows that they all have the same 11-mer binding sites, guanine (G), and uracil (U) loops at the 13th and 14th positions from the 5' end, and no G: U wobble pairing. These factors are related to the inhibition of functional mRNA expression. Additionally, the zma-miR-156 variants exhibit a single-base variation (SBV), which leads to distinct yet highly effective alterations in their interaction pattern with the XO mRNA transcript and the corresponding free energy values.

Conclusion: Therefore, we propose that zma-miR-156 variants may be a promising new bioactive compound against hyperuricemia and related diseases.

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玉米微rna作为黄嘌呤氧化酶抑制剂的计算机预测：治疗高尿酸血症患者的新途径。

简介：高尿酸血症的特征是体内尿酸（UA）升高。阻断黄嘌呤氧化酶（XO）的能力是检查不同生物活性分子如何影响高尿酸血症的有用方法。已有报道表明玉米具有抗xo活性，对高尿酸血症有显著的防治作用。人类稳定的玉米miRNA （zma-miR）的鉴定为推测其在调节新的人类基因靶点中的作用开辟了新的途径。目的：本研究旨在探讨zma-miR-XO基因调控的前景、可能的机制以及zma-miR-XO mRNA转录物的相互作用分析。方法：使用两种流行的miRNA目标预测软件intarna（版本3.3.1）和RNA杂交（版本2.2.1）揭示miRNA- mrna相互作用的显著特征。结果：在miRNA数据库中报道的325个zma-miR序列中，只有12个zma-miR-156变体有效地与XO基因的3'UTR相互作用。miRNA-mRNA相互作用的特征如下：zma-miR-156变体的定位表明它们都具有相同的11-mer结合位点，鸟嘌呤(G)和尿嘧啶(U)环位于5'端第13和第14个位置，没有G: U摆动配对。这些因素与抑制功能性mRNA表达有关。此外，zma-miR-156变体表现出单碱基变异（SBV），这导致它们与XO mRNA转录物的相互作用模式和相应的自由能值发生明显但非常有效的改变。结论：因此，我们认为zma-miR-156变体可能是一种有前景的抗高尿酸血症及相关疾病的新型生物活性化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.