Thermal gradient ring for analysis of temperature-dependent behaviors involving TRP channels in mice.

IF 2.6 4区 医学 Q2 PHYSIOLOGY
Journal of Physiological Sciences Pub Date : 2024-01-01 Epub Date: 2025-01-02 DOI:10.1186/s12576-024-00903-w
Tomoyo Ujisawa, Jing Lei, Makiko Kashio, Makoto Tominaga
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引用次数: 0

Abstract

There are a lot of temperature-sensitive proteins including transient receptor potential (TRP) channels. Some TRP channels are temperature receptors having specific activation temperatures in vitro that are within the physiological temperature range. Mice deficient in specific TRP channels show abnormal thermal behaviors, but the role of TRP channels in these behaviors is not fully understood. The Thermal Gradient Ring is a new apparatus that allows mice to freely move around the ring floor and not stay in a corner. The system can analyze various factors (e.g., 'Spent time', 'Travel distance', 'Moving speed', 'Acceleration') associated with temperature-dependent behaviors of TRP-deficient mice. For example, the Ring system clearly discriminated differences in temperature-dependent phenotypes between mice with diabetic peripheral neuropathy and TRPV1-/- mice, and demonstrated the importance of TRPV3 in temperature detection in skin. Studies using the Thermal Gradient Ring system can increase understanding of the molecular basis of thermal behaviors in mice and in turn help develop strategies to affect responses to different temperature conditions in humans.

热梯度环用于分析小鼠TRP通道的温度依赖行为。
温度敏感蛋白包括瞬时受体电位(TRP)通道。一些TRP通道是温度受体,在体外具有特定的激活温度,在生理温度范围内。缺乏特定TRP通道的小鼠表现出异常的热行为,但TRP通道在这些行为中的作用尚不完全清楚。热梯度环是一种新的装置,它允许老鼠在环地板上自由移动,而不是呆在角落里。该系统可以分析与trp缺乏小鼠的温度依赖行为相关的各种因素(例如,“花费时间”、“行进距离”、“移动速度”、“加速度”)。例如,Ring系统明确区分了糖尿病周围神经病变小鼠和TRPV1-/-小鼠之间温度依赖性表型的差异,并证明了TRPV3在皮肤温度检测中的重要性。使用热梯度环系统的研究可以增加对小鼠热行为的分子基础的理解,进而有助于制定影响人类对不同温度条件的反应的策略。
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来源期刊
CiteScore
4.40
自引率
4.30%
发文量
27
审稿时长
6-12 weeks
期刊介绍: The Journal of Physiological Sciences publishes peer-reviewed original papers, reviews, short communications, technical notes, and letters to the editor, based on the principles and theories of modern physiology and addressed to the international scientific community. All fields of physiology are covered, encompassing molecular, cellular and systems physiology. The emphasis is on human and vertebrate physiology, but comparative papers are also considered. The process of obtaining results must be ethically sound. Fields covered: Adaptation and environment Autonomic nervous function Biophysics Cell sensors and signaling Central nervous system and brain sciences Endocrinology and metabolism Excitable membranes and neural cell physiology Exercise physiology Gastrointestinal and kidney physiology Heart and circulatory physiology Molecular and cellular physiology Muscle physiology Physiome/systems biology Respiration physiology Senses.
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