Periodontitis Exacerbates Colorectal Cancer by Altering Gut Microbiota-Derived Metabolomics in Mice.

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research Pub Date : 2025-01-22 DOI:10.1111/jre.13380

Xiaoxue Wang, Zhichao Li, Haiquan Zhou, Qianyi Liu, Xueyang Zhang, Fei Hu

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引用次数: 0

Abstract

Aim: The correlation between periodontitis and colorectal cancer (CRC) has drawn widespread attention. However, how periodontitis affects CRC progression remains unclear.

Methods: C57BL/6 mice were used to establish experimental periodontitis and CRC model. Histological alterations of periodontium and colon were observed by hematoxylin and eosin staining. Micro-computed tomography (micro-CT) was applied to evaluate alveolar bone loss (ABL). Tumor growth was detected by immunofluorescence. Gut bacteria were analyzed using 16S rRNA sequencing. Gas chromatography-mass spectrometry (GC-MS) was performed to observe the alterations of gut microbial metabolites. The detection of associated pathways was carried out using quantitative real-time PCR (qRT-PCR).

Results: Experimental periodontitis significantly induced increases in tumor number in mice with CRC. Double immunofluorescence for Ki67 and β-catenin, as well as Cyclin D1 and β-catenin, indicated that experimental periodontitis observably promoted tumor growth. 16S rRNA sequencing and untargeted metabolomics analysis displayed that experimental periodontitis altered gut microbial community and metabolite profiles in CRC mice. Notably, we found that experimental periodontitis dramatically increased the level of three oncometabolites (serotonin, adenosine, and spermine) in mice with CRC.

Conclusion: Alterations of gut microbial community and metabolites might be relevant in experimental periodontitis deteriorating CRC.

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牙周炎通过改变小鼠肠道微生物衍生代谢组学使结直肠癌恶化。

目的：牙周炎与结直肠癌（CRC）的相关性已引起广泛关注。然而，牙周炎如何影响结直肠癌的进展仍不清楚。方法：采用C57BL/6小鼠建立实验性牙周炎和结直肠癌模型。苏木精染色、伊红染色观察牙周组织及结肠组织学改变。应用微计算机断层扫描（micro-CT）评价牙槽骨丢失（ABL）。免疫荧光法检测肿瘤生长情况。采用16S rRNA测序对肠道细菌进行分析。采用气相色谱-质谱法（GC-MS）观察肠道微生物代谢物的变化。采用实时荧光定量PCR （qRT-PCR）检测相关通路。结果：实验性牙周炎显著诱导结直肠癌小鼠肿瘤数量增加。Ki67和β-catenin以及Cyclin D1和β-catenin的双免疫荧光检测表明，实验性牙周炎明显促进肿瘤生长。16S rRNA测序和非靶向代谢组学分析显示，实验性牙周炎改变了结直肠癌小鼠的肠道微生物群落和代谢物谱。值得注意的是，我们发现实验性牙周炎显著增加了结直肠癌小鼠体内三种肿瘤代谢物（血清素、腺苷和精胺）的水平。结论：肠道微生物群落和代谢物的改变可能与实验性牙周炎恶化的结直肠癌有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.