Development of highly sensitive lateral flow immunoassay using PdNPs for detection of Plasmodium species

IF 3.2 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2025-01-20 DOI:10.1016/j.cca.2025.120149

Kashyap Pandya , Preeti Sharma , Maulik Rachh , Jayendra Patel

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引用次数: 0

Abstract

A lateral flow immunoassay (LFIA) employing palladium nanoparticles (PdNPs) labelled with antibodies has been innovatively designed for the precise detection of Plasmodium falciparum pLDH and HRPII antigen. This study focuses on development of LFIA based on PdNPs detection system to substantially enhance the visual detectability (vLOD), achieving an impressive 12 parasites/microliter (p/µl) vLOD in comparison with conventional system represented 50 p/µl vLOD. The research introduces a novel amplification system that not only heightens the sensitivity of LFIA but also maintains intense coloration. This novel system relies on direct detection of the malaria pLDH and HRPII antigen. In this innovative assay, HRPII antigen interacts with PdNP-conjugated Anti-HRPII detector antibodies, forming an immune complex with Anti-HRPII capture antibodies. The detection limit for HRPII antigen was found to be 8 times higher than that of conventional systems. Moreover, the novel approach for synthesis of PdNPs and their conjugation with antibodies makes this system robust and universally applicable and also extends its potential use to detect various other compounds, including antigens or antibodies from different diseases. This sensitive LFIA detection system presents itself as an efficient screening tool for medical monitoring and point-of-care (POC) settings, offering promising prospects for enhancing disease diagnosis and surveillance.

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.