Comparison of urinary cortisol, urinary cortisol-to-creatinine ratio, and basal serum cortisol as screening tests for hypoadrenocorticism in dogs.

IF 1.3 3区农林科学 Q2 VETERINARY SCIENCES

American journal of veterinary research Pub Date : 2025-01-21 DOI:10.2460/ajvr.24.10.0296

Federico Fracassi, Alessandro Tirolo, Matteo Galeotti, Andrea Corsini, Andrea Bertolazzi, Amtonio M Tardo, Stefania Golinelli, Walter Bertazzolo, Ugo Bonfanti, Fabio Procoli, Francesca Del Baldo

{"title":"Comparison of urinary cortisol, urinary cortisol-to-creatinine ratio, and basal serum cortisol as screening tests for hypoadrenocorticism in dogs.","authors":"Federico Fracassi, Alessandro Tirolo, Matteo Galeotti, Andrea Corsini, Andrea Bertolazzi, Amtonio M Tardo, Stefania Golinelli, Walter Bertazzolo, Ugo Bonfanti, Fabio Procoli, Francesca Del Baldo","doi":"10.2460/ajvr.24.10.0296","DOIUrl":null,"url":null,"abstract":"Objective: This study investigates whether urinary cortisol (UC) and UC-to-creatinine ratio (UCCR) perform better than basal serum cortisol (BSC) in identifying dogs with hypoadrenocorticism (HA).Methods: A retrospective, multicenter study with 120 client-owned dogs included: 20 with HA, 42 healthy, and 60 with diseases mimicking HA. The UC and UCCR were determined on urine samples using a chemiluminescent enzyme immunoassay. The diagnostic performance of the UC and UCCR were assessed based on receiver operating characteristics (ROC) curves.Results: A cutoff value of UC < 2 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 90.0% specificity (95% CI, 79.5 to 96.2) in diagnosing HA. A cutoff value of UCCR < 8.5 X 10-6 revealed 100% sensitivity (95% CI, 83.1 to 100) and 71.7% specificity (95% CI, 58.6 to 82.6) in diagnosing HA. A cutoff value of BSC < 2 µg/dL and < 1 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 51.7% specificity (95% CI, 38.5 to 64.8) and 100% sensitivity (95% CI, 83.9 to 100) and 90% specificity (95% CI, 79.8 to 95.3) in diagnosing HA, respectively.Conclusions: BSC < 1 µg/dL showed the same sensitivity but higher specificity than BSC < 2 µg/dL. The UC < 2 µg/dL showed noninferior performance compared with the BSC < 1 µg/dL.Clinical relevance: UC should be considered a promising screening test for canine HA.","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-7"},"PeriodicalIF":1.3000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.10.0296","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: This study investigates whether urinary cortisol (UC) and UC-to-creatinine ratio (UCCR) perform better than basal serum cortisol (BSC) in identifying dogs with hypoadrenocorticism (HA).

Methods: A retrospective, multicenter study with 120 client-owned dogs included: 20 with HA, 42 healthy, and 60 with diseases mimicking HA. The UC and UCCR were determined on urine samples using a chemiluminescent enzyme immunoassay. The diagnostic performance of the UC and UCCR were assessed based on receiver operating characteristics (ROC) curves.

Results: A cutoff value of UC < 2 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 90.0% specificity (95% CI, 79.5 to 96.2) in diagnosing HA. A cutoff value of UCCR < 8.5 X 10-6 revealed 100% sensitivity (95% CI, 83.1 to 100) and 71.7% specificity (95% CI, 58.6 to 82.6) in diagnosing HA. A cutoff value of BSC < 2 µg/dL and < 1 µg/dL revealed 100% sensitivity (95% CI, 83.2 to 100) and 51.7% specificity (95% CI, 38.5 to 64.8) and 100% sensitivity (95% CI, 83.9 to 100) and 90% specificity (95% CI, 79.8 to 95.3) in diagnosing HA, respectively.

Conclusions: BSC < 1 µg/dL showed the same sensitivity but higher specificity than BSC < 2 µg/dL. The UC < 2 µg/dL showed noninferior performance compared with the BSC < 1 µg/dL.

Clinical relevance: UC should be considered a promising screening test for canine HA.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

American journal of veterinary research 农林科学-兽医学

CiteScore

1.70

自引率

10.00%

发文量

186

审稿时长

3 months

期刊介绍： The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.