Certified reference materials for GMO analysis—more than 25 years of GMO CRM production at EC JRC

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-01-23 DOI:10.1007/s00216-024-05713-y

Stefanie Trapmann, Thomas P. J. Linsinger, Robert Koeber

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引用次数: 0

Abstract

Certified reference materials (CRMs) play a crucial role in ensuring the quality of analytical measurements. Particularly, the EU labelling legislation on genetically modified organisms (GMOs) in food and feed products explicitly requires CRMs for its implementation. The CRMs are used to calibrate and control the quantitative real-time polymerase chain reaction (qPCR) method and support official control laboratories, such as National Reference Laboratories (NRLs), in maintaining their ISO/IEC 17025 accreditation. The Joint Research Centre of the European Commission (EC JRC) is specialized in the production of reference materials and has been producing GMO CRMs since 1998. Together with a validated event-specific qPCR method, these GMO CRMs form the EU reference system for the quantification of EU-authorized GMO events in food and feed products and ensure a common GMO labelling threshold. This article gives a retrospective view on the more than 25 years of GMO CRM production at JRC. It describes requirements for GMO CRMs in view of an EU market authorization under (EC) No. 1829/2003. The evolution and major improvements of the production of GMO CRMs at JRC are summarized as well as the current understanding of the EU’s GMO reference system for GMO quantification and its impact on commutability. It provides insights into GMO CRM sales and their worldwide distribution. This information may be useful for policymakers and researchers in understanding the current EU GMO measurement landscape and to anticipate possible future demands related to GMO events based on new genomic techniques (NGTs).

查看原文本刊更多论文

转基因生物分析的认证参考物质- EC JRC超过25年的转基因生物CRM生产。

经认证的标准物质（CRMs）在确保分析测量质量方面起着至关重要的作用。特别是，欧盟关于食品和饲料产品中转基因生物（GMOs）的标签立法明确要求实施crm。crm用于校准和控制定量实时聚合酶链反应（qPCR）方法，并支持官方控制实验室，如国家参考实验室（nrl），以保持其ISO/IEC 17025认证。欧盟委员会联合研究中心（EC JRC）专门从事标准物质的生产，自1998年以来一直在生产转基因生物标准物质。与经过验证的事件特异性qPCR方法一起，这些转基因生物crm构成了欧盟的参考系统，用于定量欧盟批准的食品和饲料中转基因生物事件，并确保了通用的转基因生物标签阈值。本文回顾了JRC 25多年来的转基因CRM生产情况。它描述了根据（EC） No 1829/2003的欧盟市场授权对转基因生物crm的要求。本文总结了JRC转基因生物标准物质生产的演变和主要改进，以及欧盟转基因生物量化参考系统的现状及其对可交换性的影响。它提供了对GMO CRM销售及其全球分销的见解。这些信息可能有助于政策制定者和研究人员了解欧盟当前的转基因生物测量情况，并预测基于新基因组技术（NGTs）的转基因生物事件可能带来的未来需求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.